For a reporter protein to be synthesized its gene

9. For a reporter protein to be synthesized, its gene must be fused in the proper codon reading frame with respect to the
a. ribosome binding site.
b. promoter of the gene of interest.
c. vector origin of replication.
d. target gene.
e. vector’s antibiotic marker sequence.
10. What kind of gene expression controls are revealed by operon fusions?
11. What type of reporter fusions can expose both transcriptional and translational controls?
12. Which of the following is correct regarding operon fusions?

13. What is the main indication that the figure below depicts a transcriptional (operon) fusion?
a. A single mRNA is transcribed, from which two proteins are translated.
b. The reporter gene keeps its biological properties: in this case, fluorescence.
c. Transcription proceeds from a single promoter.
d. The target gene maintains its ribosomal binding site.
e. The reporter gene is inserted into the target gene in the proper codon-reading frame.
14. Sigma B is a stress-induced transcription factor in Bacillus subtilis. Bacteria producing a sigma B-YFP fusion protein were exposed
to constant energy stress, and fluorescence pulses emitted by individual cells were detected by time-lapse microscopy. Could this
result have been obtained using a sigma B-b-gal fusion protein?