2 The yellow strand is Website wwwdnaiorg Code Copying the Code Putting it

2 the yellow strand is website wwwdnaiorg code

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2. The yellow strand is ___________________. Website: Code Copying the Code Putting it Together Interactive 3. The first nucleotide in the RNA strand that you synthesized was ______. 4. The last nucleotide in the RNA strand that you synthesized was ______. Part III: Transcribe & Translate a Gene Website: learn.genetics.utah.edu Basic Genetics Transcribe & Translate a Gene 1. The start codon is _________. 2. The first amino acid is ___________________. 3. The last amino acid in the polypeptide chain you synthesized is _________. 4. The stop codon for this exercise is _________.
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31 Lecture 6: Exam 1 Review Complete this study guide to review for Exam 1. 1. What are the applications of molecular testing? 2. Briefly describe the scientific accomplishments of: a. Mendel b. Miescher c. Griffith d. Avery, MacLeod, & McCarty e. Chargaff f. Franklin & Wilkins g. Watson & Crick 3. Compare & contrast DNA & RNA. DNA RNA Nitrogen bases Pentose sugar Location Stability Relative nucleotide length Typically double- stranded or single- stranded? Helical shape? Function 4. Know the types of RNA & their function: mRNA, rRNA (ribosomes), tRNA. 5. Nitrogenous bases: a. Which are purines, which are pyrimidines? b. Structure (single ring, double ring) c. What substitution in RNA? d. Which ones pair up & how many hydrogen bonds are formed between them? 6. Composition & structure of nucleoside & nucleotide. Be able to identify from a diagram.
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32 7. Basic DNA structure what does anti-parallel mean? Complementary? a. What is the unit of measure for DNA? 8. DNA/RNA sequences are ALWAYS read in what manner? 9. Know the key enzymes involved in DNA replication & their function. 10. What is a restriction enzyme? 11. What are the three basic steps in nucleic acid extraction? 12. Given the steps for liquid-phase or solid-phase extraction, could you put them in order? 13. Be able to determine purity & yield of DNA when given OD or absorbance readings. 14. Summarize transcription & translation. Transcription Translation Where does it occur? Brief description End result DNA directly involved? RNA type(s) involved? 15. What are introns & exons? 16. Given an mRNA codon sequence, determine what protein is coded for using the mRNA codon chart. Also determine the tRNA anti-codon sequence. 17. Terms to know: a. Gene b. Genome c. Phenotype d. Genotype
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33 Lecture 7, Part I: Nucleic Acid Amplification by PCR Objectives Following the lecture the student will be able to: 1. List the main components in PCR reactions. 2. Briefly describe the characteristics of Taq DNA polymerase including its original source and optimal temperature. 3. Summarize the three major steps in the PCR process. 4. Define temperature cycle and amplicon. 5. State the approximate amount of time for one complete temperature cycle. 6. Describe the function of thermal cyclers. 7. Summarize the types and relative number of DNA sequences present in a reaction tube after the completion of a PCR process.
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