Aerotolerant anaerobes a Typically lack catalase but have SOD Obligate

Aerotolerant anaerobes a typically lack catalase but

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Aerotolerant anaerobes: a) Typically lack catalase but have SOD Obligate anaerobes: a) Typically lack both catalase and SOD, because they cannot tolerate oxygen Most common toxic oxygen species
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at all, so they proceed via superoxide reductase which reduces toxic oxygen to H₂O₂ without O₂ production, avoiding exposure to the organism H₂O₂ isn’t as toxic as O₂⁻ and OH• If there’s a problem with one enzyme in the cascade of reactions to break down the relative form toxic oxygen, it will lead to excess in the cell, which is bad. Culture Conditions Aerobes need extensive aeration a) Vigorous shaking of flasks or tubes b) Bubbling sterilized air through medium Anaerobes cannot have any trace of oxygen a) Add reducing agent Such as thioglycolate (medium contains small amount of agar) Can also use H 2 S – it removes O₂ in the form of H₂O b) Anoxic Jar / Envelope (gas pak) uses H 2 + CO 2 + palladium catalyst H 2 reacts with oxygen, removing it H 2 + O 2 H 2 O c) Anoxic Glove Box Air can be evacuated and filled with O 2 free gas Anoxic Jar Anoxic Glove Box
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