Double check by holding your ygc plate at an angle to

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YGC Plate. Double-check by holding your YGC Plate at an Angle to the Light. If the Oddball Colony is not growing on a Streak Line on your Plate, it’s almost certainly a Contaminant from the Air. All Species of Micrococcus are Non-Pathogenic, so you can separate these from your “Patient” Isolate and continue to grow them if you’d like. It is also possible that a Clan will “isolate” Staphylococcus aureus as a Contaminate from a Clan Member’s Skin by leaving your YGC Plate uncovered for too long while you were streaking, allowing a Hair or some dead Skin Cells (and Staphylococcus aureus ) from that Clan Member to settle onto your YGC Plate. Again, check if the Oddball Colony isn’t growing on a Streak Line on your Plate. Regardless, Staphylococcus aureus is a Pathogen and it is responsible for all sorts of nasty Infections of the Skin. Do not restreak any Colonies you suspect could be Staphylococcus aureus and put any Plates contaminated with Staphylococcus aureus into Oscar.
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Lab 1 Page 31 Gameplan for the Isolation of Enteric Bacteria (E. coli and Enterobacter sp.) E. coli and Enterobacter aerogenes are Gram Negative, Non-Spore-Forming Rods that are Motile by means of Peritrichous Flagella. They are Facultative Aerobes. Note: Enteric Bacteria (or simply Enterics) refers to a Group of Gram Negative, Oxidase Negative Bacteria found in the Intestinal Tract (Enteric Tract). Enterobacter sp. is a Genus of Enteric Bacteria. E. coli is one of the most intensively studied of all Species. It has been the Lab Rat for Classical Experiments in Microbiology and Molecular Biology. All Escherichia in Nature are entirely Symbiotic, occurring in large Numbers in the Gut of Warm-Blooded Animals. Escherichia is not normally Pathogenic, but can cause serious Infections when it gains Access to the Bladder. E. coli can also cause Peritonitis if the Intestine is punctured -- say by a Gun or Knife Wound -- and the Intestinal Contents are released into the Peritoneal Cavity. You should treat Escherichia as a Pathogen. Enterobacter is widely distributed in Nature as both Free-Living and Symbiotic Strains, and is often found in large Numbers in decaying Organic Material. E. coli and Enterobacter are important in Water Quality Testing. The two must be distinguished: Enterobacter is a normal Inhabitant of the Soil and Water; E .coli does not naturally occur in either Soil or Water and its Presence is an Indicator of Fecal Contamination and the Presence of more serious Pathogens. These two Genera can be distinguished from other Intestinal Bacteria by their Ability to ferment Lactose (with the Production of Hydrogen). This Ability is not very widespread among Bacteria, so Media containing Lactose as the Carbon Source is an easy Way to detect the Presence of these Bacteria. We’ll do this as Part of Water Quality Testing in a later Lab.
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