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Each of these dots is called a colony and each

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small Button on the Petri Plate. Each of these Dots is called a Colony and each isolated Colony represents a Pure Culture of that Bacterium. We will be using Streak Technique to obtain Pure Cultures of Bacteria. Streak Technique involves using a Sterile Aluminum Loop to transfer Bacteria from one Plate to another Plate: (A) Without contaminating the Lab with the Bacteria on these Plates, and (B) Without contaminating these Plates with the Bacteria in the Lab. Streaking is a critically important Skill to master; it’s also a surprisingly easy Technique to learn. Incubation After you’ve streaked your Plates we’ll put them in the appropriate Incubator. We have two Incubators (30°C and 37°C) and a Walk-In Cold Room (04° C). If it comes from a Human Source, it should be incubated in the 37°C Incubator. Stuff that comes from a Human Source would include E. coli , Enterobacter aerogenes , Micrococcus luteus and Staphylococcus epidermidis . Generally speaking, Stuff that goes in the 37°C Incubator is usually -- but not always -- incubated for 24 Hours. If it comes from Dirt, it should be incubated in the 30°C Incubator. Stuff that comes from Dirt would include Bacillus and Pseudomonas . Stuff that goes in the 30°C Incubator is usually -- but not always --incubated for 48 Hours. After Incubation, Cultures are usually moved into the Cold Room (04°C) until you need them for Lab. Most Bacteria can survive in the Cold Room for several Weeks. ____________________________________________ “They let us go. It was the only reason for the ease of our escape.” Easy? You call that easy?’ They're tracking us.” Not this ship, sister.’ At least the information in R2 is still intact.” What's so important? What's he carrying?’ The technical readouts of that battle station. I only hope that when the data's analyzed a weakness can be found. It's not over yet.” © 1977 Lucasfilm, Ltd.
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