Aperture the hole in the stage that allows light

This preview shows page 2 - 4 out of 9 pages.

Aperture: the hole in the stage that allows light through for better viewing of the specimen. Parfocal microscope objectives: The specimen being observed remains in focus when magnification is changed; i.e., if the microscope is switched from a higher power objective (e.g., 40×) to a lower power objective (e.g., 10×), the specimenremains in focus.Field of View: Sometimes abbreviated "FOV", it is the diameter of the circle of light that you see when looking into a microscope. As the power gets greater, the field of view gets smaller. Complete the Table 1 in the Lab report for calculations of magnification and identifying parts of microscope.Exercise 2: Observation of Different Types of BacteriaMaterials:Compound microscope, immersion oil, lens wipes, prepared slides of various bacteria- Rods, cocci and spiralNotes to Students:For this exercise, you may have a single slide containing three different types of bacteria or you may have three slides, each containing only one type of bacteria. Check with your instructor about how many slides you will be observing.Method: 1.Obtain the preserved slide of a bacteria.2.Use Appendix A and follow the instructions on how to use a Microscope.3.Observe the slide under 10X, and then 40X objective lenses. 4.Record and describe your observations made using 40X lens.5.Make sure to note as many details as possible about the size, shape and color (stain) of bacteria.6.Use 100X oil immersion lens if needed and record your observations.Note to Students:Don’t forget to use oil with 100X lens only. After completing the observation, clean the oil immersion lens with lens wipes to remove oil. Be careful to not contaminate other lenses (4X, 10X, 40X) with oil as this will cause damage to the other lenses.7.Repeat the steps 1-6 for slides containing bacteria of different shapes as needed. Record your observations in thelab report. Exercise 3: Observation of Eukaryotic Cells- Protozoa and FungiMaterials:Compound Microscope, prepared slides of Protozoa and fungus Method: Protozoa:2
BIOS242, Week 2, Lab 1Name: 1.Obtain the preserved slide of a protozoa.2.Use Appendix A and follow instructions on how to use a Microscope.3.Observe the slide using 10X, and 40X objective lenses. 4.Record and describe your observations made using 40X in the Lab report. Make sure to note as many details as possible about the size, shape and color (stain) of protozoa.Fungi:1.Obtain the preserved slide of a multicellular fungi.2.Use Appendix A to follow instructions on using a Microscope.3.Observe the slide using 10X, and 40X objective lenses. 4.Record and describe your observations made using 40X in the Lab report. Make sure to note as many details as possible about the size, shape and color (stain) of fungus.

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture