Dna bacteriophage replication phage attaches to

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DNA Bacteriophage Replication • Phage attaches to Bacterium and injects its DNA With some DNA Phage, once the DNA enters the Cytoplasm it behaves exactly like a Plasmid (think F Factor); it can remain Autonomous in the Cytoplasm, or it can integrate into the Bacterium ʼ s Chromosome. Remaining in the Cytoplasm is a Safe Strategy. Each Phage will produce ~100 progeny Phage. Integrating into the Bacterium ʼ s Chromosome is risky. You ʼ re joining your Genetic Fate to that of the Bacterium. But… it means roughly once an Hour you ʼ ll be replicated along with the Bacterial Chromosome. So eventually, each Phage could possibly produce Millions of progeny Phage. • Phage DNA commandeers Bacterial Replication, Transcription and Translation Mechanisms - Bacterium is “retooled” to -- • Transcribe Phage DNA into Phage mRNA • Translate Phage mRNA into Phage Proteins • Replicate Phage Nucleic Acid, and • Phage Proteins assemble into Capsids • Phage DNA is inserted into Capsids • Phage lyses Host Bacterium to release progeny (L= descendent) Phage to attack other Bacteria Transduction is basically a Packaging Mistake • Most Phage package DNA based on Length, not specific Sequences • Bacterial DNA can be packaged into the Phage Capsids - So some progeny Phage may contain Bacterial Genes • When such progeny Phage infects another Bacterium it will inject Bacterial Genes The Bacterium can “sample” alternative Versions of Genes transduced by Bacteriophage. Transduction is possibly the most utilized form of Gene Transfer among Bacteria
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