6. Regarding the effect of pH on enzyme activity, which is the dependentvariable? A.temperatureB.pHC.amount of substrate (sucrose) presentD.amount of product (glucose and fructose) presentAnswer: D7. Regarding the effect of temperature on enzyme activity, which is the independentvariable?8. When looking at the effect of pH on sucrase activity, what was the wavelength of light used in the spectrophotometer? 9. When looking at the effect of temperature on sucrase activity, the pH was set to which number? Why was this number chosen?10. How did you determine the average value for a pH of 3 in Table 4?
STOP!!Remember, you will complete questions from thisassignment sheet only. You will NOTfill out or submit the online lab report.Lab reportAnswer the following two questions by referring to the graph above.11. What is the optimum pH for sucrase activity?
12. What happens to the activity of sucrase as the pH becomes more alkaline? Section 5 - short answer (5 pts)During chemistry lab, Maria places sucrose in a glass beaker, adds water, and stirs. As the table sugar disappears, she loudly proclaims that she has chemically broken down the sucrose into fructose and glucose. Is Maria's chemical analysis correct? Why or why not?
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