Using the uniprot data base it was found that some of

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known human proteins found on Figure 1 page 33 of the laboratory manual. Using the UniProt data base it was found that some of the Bovine proteins were the same as humans. Protein 1(144.93 kDa) was identified as Palladin, also referred to as Band 3 (150.56 kDa). Palladin works in the cell to improve mobility, adhesion and also plays a role in cell-cell interactions (Palladin, 2015). Protein 2 (97.08 kDa) was identified as Band 4.1- Protein 1 (98.08 kDa). The function of this protein is to create stability and elasticity in integrate membranes and channels (Band 4.1, 2015). Protein 3 (44.63 kDa) was determined to be Dematin 4.9 (45.514 kDa). The role of Dematin in the cell is help maintain and regulate cell shape and functionality (Dematin, 2015). The final unknown protein, protein 4 (19.52 kDa) will remain unidentified due to the fact all the human proteins outlined in the laboratory manual do not have a similar molecular weight. Thus, the hypothesis that Bovine blood cells will have proteins in common with human blood cells, is supported. A possible explanation to why protein 4 was not identified may be a result of some error. Specifically several sources of error are present in this study. To being, the sample protein bands were relatively unclear in the gels and difficult to interpret. This may have resulted in the protein bands begin measure inaccurately and resulting protein misidentification. Secondly, when comparing the Bovine proteins to human proteins the molecular weights were not an exact match. For example, Protein 1 was measure to be 144.93kDa and this was assumed to be
SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS OF BOVINE BLOOD PROTEINS 10 Palladin which has a molecular mass of 150.56 (Palladin, 2015). Here the molecular weights differ by 5.6 kDa which may have resulted once again in misidentification. If this study was to be conducted again more precise and accurate methods and practices should be used to ensure the proteins are being correctly identified. In terms of real world relevance, studies such as this are important when looking at various illnesses, and diseases. Specifically, one study looked at the malaria parasite. The malaria parasite secretes proteins into their host cells, which then effects and inhibit proteins found in red blood cell membranes (An, & Mohandas, 2012). As a result blood cells began to lose shape, adhesiveness and eventually erupted completely and cause the organism to be highly anemic (An, & Mohandas, 2012). By conducting similar experiments we are able to understand vital protein functions and potentially develop treatments for when these proteins are damaged or inhibited. In conclusion, similarities do exist between Bovine and human proteins. This premise can be useful in future research specifically, in the medical field.
SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS OF BOVINE BLOOD PROTEINS 11 References Alberts, Bray, Hopkin, Johnson, Lewis, Raff, Roberts & Walter. (2014) Essential Cell Biology, 4 th Edition. Garden Science : New York.

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