bag in Table 3. Seal the dialysis tubing with the remaining green rubber band. Rinse and
dry the graduated cylinder.
16. Place the yellow, red, and blue banded tubing in Beaker 2 (3% sucrose solution). Place
the green banded tubing in Beaker 1 (30% sucrose solution). See Figure 8. Record the %
sucrose of the beaker that each tubing is placed into in Table 3.
Hint
: Covering the beakers with a sheet of Saran wrap or aluminum foil helps keep the
top of the dialysis tubing from drying out.
Figure 8:
The dialysis bags are filled with varying
concentrations of sucrose solution and placed in one
of two beakers.
17. Hypothesize whether water will flow in or out of each dialysis bag. Include your
hypotheses, along with supporting scientific reasoning in the Hypotheses section at the
end of this procedure.
18. Allow the bags to sit for one hour.
19. After allowing the tubing to sit for one hour, remove them from the beakers.
20. Carefully open the tubing. The top of the tubing may need to be cut off/removed as it
tends to dry out over the course of an hour. Measure the solution volumes of each dialysis
bag using the 10 mL and 100 mL graduated cylinder. Make sure to empty and dry the
cylinder completely between each sample.
21. Complete Table 3 by calculating the net displacement of water from each piece of
dialysis tubing.
Table 3: Sucrose Concentration vs. Tubing Permeability
Band Color
Sucrose
% in
Beaker
Sucrose
% in Bag
Initial Volume
(mL)
Final Volume
(mL)
Net Displacement
(mL)
Yellow
Red
Blue
Green
