Seal the dialysis tubing with the remaining green rubber band Rinse and dry the

Seal the dialysis tubing with the remaining green

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bag in Table 3. Seal the dialysis tubing with the remaining green rubber band. Rinse and dry the graduated cylinder. 16. Place the yellow, red, and blue banded tubing in Beaker 2 (3% sucrose solution). Place the green banded tubing in Beaker 1 (30% sucrose solution). See Figure 8. Record the % sucrose of the beaker that each tubing is placed into in Table 3. Hint : Covering the beakers with a sheet of Saran wrap or aluminum foil helps keep the top of the dialysis tubing from drying out. Figure 8: The dialysis bags are filled with varying concentrations of sucrose solution and placed in one of two beakers. 17. Hypothesize whether water will flow in or out of each dialysis bag. Include your hypotheses, along with supporting scientific reasoning in the Hypotheses section at the end of this procedure. 18. Allow the bags to sit for one hour.
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19. After allowing the tubing to sit for one hour, remove them from the beakers. 20. Carefully open the tubing. The top of the tubing may need to be cut off/removed as it tends to dry out over the course of an hour. Measure the solution volumes of each dialysis bag using the 10 mL and 100 mL graduated cylinder. Make sure to empty and dry the cylinder completely between each sample. 21. Complete Table 3 by calculating the net displacement of water from each piece of dialysis tubing. Table 3: Sucrose Concentration vs. Tubing Permeability Band Color Sucrose % in Beaker Sucrose % in Bag Initial Volume (mL) Final Volume (mL) Net Displacement (mL) Yellow Red Blue Green
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