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rubber band. 10 mL of the 3% sucrose solution in the bag with the blue rubber band, and seal the dialysis tubing with the remaining blue rubber band. The final 10 mL of 3% sucrose solution in the bag with the green rubber band. Seal the dialysis tubing with the remaining green rubber band.
Figure 5: The dialysis bags are filled with varying concentrations of sucrose solution and placed in one of two beakers.13. Verify and record the initial volume of solution from each bag in Table 6. 14. Place the yellow, red, and blue banded tubing in Beaker 2. Place the green banded tubing in Beaker 1 (Figure 5). 15. Hypothesize whether water will flow in or out of each dialysis bag. Include your hypotheses, along with supporting scientific reasoning in the Hypotheses section at the end of this procedure. 16. Allow the bags to sit for one hour. While waiting, pour out the water in the 250 mL beaker that was used to soak the dialysis tubing in Step 1. You will use the beaker in Step 19. 17. After allowing the tubing to sit for one hour, remove them from the beakers. 18. Carefully open the tubing. The top of the tubing may need to be cut off/removed as they tend to dry out over the course of an hour. Measure the solution volumesof each dialysis bag using the 100 mL graduated cylinder. Make sure to empty and dry the cylinder completely between each sample. 19. Record your data in Table 6. Hypothesis: