Lab__3a_Isolation_Streak_Plate_Method

To help identify our unknown we will culture it onto

Info icon This preview shows pages 3–5. Sign up to view the full content.

View Full Document Right Arrow Icon
To help identify our unknown we will culture it onto MacConkey’s & Mannitol Salt using streak plate method . To do a streak plate technique , we will use an inoculation loop ( aka smear loop, inoculation wand or microstreaker). Simple tool used to retrieve an inoculum from a culture of microorganisms. Always sterilize in microincinerator until loop becomes red hot before and after each use. By doing this, the same tool can be reused in different experiments without fear of cross-contamination. Be sure that your inoculation loop has cooled before using it to retrieve inoculum or to streak a plate! If you hear medium sizzle when you touch it with loop , the loop is too hot! Images:; Isolation streak plate of Legionella , PHIL #7925 Inoculation loop , Jeffrey M. Vinocur; Microincinerator, T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.com
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
When obtaining a bacterial sample from a tube or plate of media do so gently ! The bacteria is growing as a thin film on top of the media! Don’t scrape so hard that you
Image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern