To help identify our unknown we will culture it onto

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To help identify our unknown we will culture it onto MacConkey’s & Mannitol Salt using streak plate method . To do a streak plate technique , we will use an inoculation loop ( aka smear loop, inoculation wand or microstreaker). Simple tool used to retrieve an inoculum from a culture of microorganisms. Always sterilize in microincinerator until loop becomes red hot before and after each use. By doing this, the same tool can be reused in different experiments without fear of cross-contamination. Be sure that your inoculation loop has cooled before using it to retrieve inoculum or to streak a plate! If you hear medium sizzle when you touch it with loop , the loop is too hot! Images:; Isolation streak plate of Legionella , PHIL #7925 Inoculation loop , Jeffrey M. Vinocur; Microincinerator, T. Port From the Virtual Microbiology Classroom on
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When obtaining a bacterial sample from a tube or plate of media do so gently ! The bacteria is growing as a thin film on top of the media! Don’t scrape so hard that you
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