5.During what stage of bacterial growth are cell death and cell division balanced, leading to no net change in cell number? a.Death Phase b.Exponential (Logarithmic) Phase c.Lag Phase d.Stationary phase e.Turbid Phase
6.You are trying to isolate a bacterium that can be found in the soil in very small numbers compared to the total population of all bacteria in the soil. In order to perform this isolation, you are first going to take a sample from the soil and add it into a liquid growth medium that contains a compound that only your target organism can use to grow on. What type of procedure are you performing in this first step?
7.You grow a culture of Pseudomonas putidain 5 milliliters of growth medium until there are a total of exactly 50 million cells in the tube (you’ve always been good at counting things). In order to test your dilution technique, you perform a series of dilutions by adding 1 mL of the culture into 9 mL of sterile saline, mixing, and adding 1mL of that diluted mixture into the next tube of 9 mL sterile saline. If you always spread 100 microliters (µL) of a dilution onto a plate, how many dilution tubes do you need to use to end up with 100 cells on a plate?
8.Which sample would you most want to use Phase Contrast to view?
9.While isolating bacteria in MIC103L, a common step is streaking bacteria onto a solid medium in a Petri plate in order to separate them into colonies derived from a single bacterium (and therefore generating a population of genetically-identical clones in that colony). When isolating your Patient Skin sample, you have to restreak your bacterium from a Nutrient agar plate onto a YGC plate. Once you have both plates on your bench labeled, you light your Bunsen burner and pick up your loop, ready to go. How many times do you sterilize your loop from start to finish of streaking? a.Two (2) times b.Three (3) times c.Four (4) times d.Five (5) times e.Six (6) times