BCH_3053_Spring_2015_Exam_2_Answer_Key_Fina2l (1)

A mutant form of peptide hormone angiotensin ii has

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12. A mutant form of peptide hormone angiotensin II has the amino acid composition Asp, Arg, Ile, Met, Phe, Pro, Tyr, Val. The following observations are made: Trypsin yields a peptide containing Asp and Arg, and a hexapeptide with all the rest. Trypin cleaves on the C-side of Lys and Arg. Cyanogen bromide (CNBr) cleavage yiealds a dipeptide containing Phe and Pro, and a hexapeptide containing all others. CNBr cleaves on the C-side of Met. Chymotrypsin cleaves into two tetrapeptides, of composition Asp, Arg, Tyr, Val and Ile, Met, Phe, Pro. Chymotrypsin cleaves on the C-side of Tyr, Trp, and Phe. The dipeptide of composition Pro, Phe cannot be cleaved by chymotrypsin The sequence of the peptide is:
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