Bio326RSu19.02.History.BasicMethods-4.pptx

2 2 germ theory infectious diseases are caused by

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Germ Theory Infectious diseases are caused by microorganisms Pathogens : Prions and Viruses (non-living) Bacteria Fungi Parasites Each microorganism causes a particular disease in susceptible individuals The susceptibility of an individual depends on: The infecting agent Factors involving the environment Factors involving the host
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Development of Bacteriologic Techniques “Artificial” growth of microorganisms Artificial media Potatoes Gelatin, agar, and Fannie Hesse Petri plates Culture media formulations
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Solid or liquid for growth, transport, or storage of microorganisms Containers Nutrients Sometimes organism-specific Energy sources Carbon, nitrogen, phosphorous, sulfur, minerals Oxygen Usually sterilized by boiling (autoclave) Culture Media Plate Deep E. Flask Slant 2-3
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Isolation of “Pure Cultures” Population of cells of a single species Arise from a single cell in a complex mixture of species Mixture Pure Culture Isolation
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Isolation of “Pure Cultures” Basis of pure culture isolation is the colony “Clump” of cells arising form single cell Usually grown on solid culture media Colony morphology can be species-specific
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12 STREAK PLATE TECHNIQUE FOR PURE CULTURES 1. SWAB 2. STERILE LOOP 3. STERILIZE LOOP, STREAK AGAIN PETRI PLATE AFTER INOCULATION PETRI PLATE AFTER INCUBATION COLONY, PURE KOCH
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Colony Morphology Colonies 1, 2, 5: Cocci of different sizes, occurring singly or in small two-dimensional clusters. Colonies 3, 4, 7: Large cocci, forming tetrads. Colonies 6, 11: Large cocci with three-dimensional planes of cell division, giving rise to quite regular 'packets' of cells, typical of Staphylococcus .
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