Negative stains have a brief shelf life spore

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Negative Stains have a Brief Shelf Life. Spore Staining (5 Points) 1. Make a Spore Stain using our Bacillus Culture. 2. Once you have your Spore Stain Slide focused properly Stained Bacillus Spores under 100X Oil Immersion, have your TA evaluate your Slide. A Copy showing what the Staining Practical Answer Sheet looks like is on Page 6
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Lab 9 Page 4 Microscope Practical We will be checking your Microscope for Brightfield Alignment by -- A. Checking that your “P” is in Focus B. Checking that your Field Diaphragm (“Polygon”) is centered and in Focus C. Checking that your Condenser Diaphragm is open such that it covers about 3/4 of the Field of View. You’ll be docked 1-2 Points if your “P” is out of Focus (depending on just how badly it’s out of Focus), 1-3 Points if your Field Diaphragm is not centered or is out of Focus, and 1-2 Points if your Condenser Diaphragm is not set correctly. You’ll be docked 7 Points if your “P” is wildly out of Focus but your Field Diaphragm (“Polygon”) is in Focus. This means you used your Fine and Coarse Focus Controls -- instead of the Condenser Focus Knob -- to focus the Image of your Field Diaphragm. So don’t do this, OK? We’ll be checking that you know the Parts of the Microscope by having you identify Five Microscope Parts. We’ll have Pointers attached to Microscope Parts in the Middle Room. Face it, there just aren’t that many different Part on these Microscopes that are all that difficult to learn so concentrate on the Parts associated with the Stage and the Condenser. Staining Practical The Staining Practical is Closed Book. Please put your Atlas , your Lab Manual, your Lab Notebook and any Notes away. Please do not talk with anyone during the 90 Minute Practical. You’ll receive an Answer Sheet that has two Gram Stain Unknowns (1 and 2 or A and B) where you circle whether each Unknown is Gram Positive or Gram Negative. The Answer Sheet also has a Scale of 5 (Outstanding) to 0 (Problematic) for Negative Staining and Spore Staining. You need to show us a Negative Stain of Azomonas under 40X. We want to see individual Azomonas Capsules. You need to show us a Spore Stain of our Bacillus under 100X Oil Immersion. We want to see Bacillus Endospores with the expected Staining and Morphology.
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