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under high power. Show your work below with your estimate of the length (in micrometers) of your high powerfield of view.High Power Field of View = _______9. Sketch a paramecium and a cork slide at400x, drawing each to scale with attention to detail.Length of paramecium = ____________Length & Width of single cork cell = ____________Part 2 Analysis:1.Check that each section of this page is complete.2.Explain why you cannot use the ruler technique to estimate specimens at high power.
15Part 3: Working with SpecimensA. Making a Wet Mount of a SlidePlace a drop of water on the slide. Gather afew strandsof cottonusing forceps and place them on the drop.Place the coverslip at about an angle with one edge touching the water drop and then gently let go.Performed correctly the cover slip will perfectly fall over the specimen and will not have air bubbles. If the coverslip does not sit flat, then you have too much fiber on the slide.If this is the case, start over.Draw the specimen as it appears in your viewing field under scanning, low and high power. Draw the strandsto scale.Save the slide for part B below.B. Staining a SpecimenBiologists learn to stain specimens that have already been created. You will not remake the slide, but use aprocedure to draw a stain under the coverslip.Place one drop of stain (methylene blue or iodine) onthe edge of the coverslip you made with the cotton.Place the flat edge of a piece of paper towel on theopposite side of the coverslip. The paper towel will draw thestain across the slide due to thecohesionproperty ofwater.View the slide again, note the appearance of the cottonfibers now compared to the view without the stain.C. Viewing Large Specimens and DissectingScopesLight microscopes are only useful for viewing small thin specimens.Stereoscopespresent a larger field ofviewing and handle depth much better than the light microscope. The drawback of the stereoscope is that it doesnot have a high magnification. Examine one of the stereoscopes in the room which will have a variety ofspecimens to view.Name/Description of specimen(s) viewed: _________________________________________________
16D. Depth PerceptionObtain a slide with three different colored threads on it. View the slide under scanning and then lowpower. You should note that you could only focus on one colored thread at one time. Figure out whichthread is on top by lowering your stage all the way, then slowly raising it until the thread comes intofocus. The first thread to come into focus is the one on top. Make sure to pay attention to the workingdistance and not crush the slide (should not be a problem at scanning or low power).Which color thread is on top? _____________middle? ______________bottom?_____________Part 3 Analysis:1. Describe the difference when you viewed unstained versus stained cotton fibers.2.What are the advantages of using a stereoscope?What are the disadvantages?
17Exercise 3:Diffusion and OsmosisLaboratory ObjectivesAfter completing this laboratory topic, you should be able to:1.

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