12-13_Microbial_Growth___Metabolism_Combo

Q how do cells get energy if there is no o 2

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Unformatted text preview: Q: How do cells get energy if there is no O 2 available to them, or if they can’t use O 2 ? From the Virtual Microbiology Classroom on ScienceProfOnline.com ______________ • When there is no final __________ __________ for the ETC, the electron transport can’t happen. • Fermentation is an alternative system that allows glycolysis to continue without the other steps of respiration. • Not as energetically efficient as respiration. • Produces only 2 ATP . ATP ATP From the Virtual Microbiology Classroom on ScienceProfOnline.com Metabolism & Identification of Microbes Some of the specialized media that we have worked with in lab is both selective and differential . The differential properties give us information about bacteria based on its metabolism. Qs: What is the medium in top picture? • Is selective …Why? What does it grow? • Is differential …Why? • What does the differential property reveal about the bacteria growing there? Qs: What is the medium in bottom picture? • Is selective …Why? What does it grow? • Is differential …Why? • What does the differential property reveal about the bacteria growing there ? Images: MacConkey’s Agar (MAC) & Mannitol Salt Agar (MSA), T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.com Metabolism & Identification of Microbes API-20E • The API-20E test is used to ID Gram- negative enteric bacilli-shaped bacteria from the family. • System of 20 individual, miniaturized tests used to determine the ____________ ____________ of the organism. • Some microbes can metabolize certain molecules while others can’t. • When molecules are metabolized, specific waste products are created. • From identification of metabolic capabilities, we can zero in on identification of genus and species. Images: API- 20E with results sheet, T. Port; API- 20E results for different species of bacteria, Dr. Charles Gerba , University of Arizona From the Virtual Microbiology Classroom on ScienceProfOnline.com Microbial Growth • Refers to increase in the ____________ of microbes (reproduction) rather than an increase in ____ of the microbe. • Result of microbial growth is the ________ = aggregation of cells arising from single parent cell. • The time required for growth and reproduction is known as the doubling or ___________ ______. Image: Glowing Colony E. coli from "Aging and Death in E. coli" (2005) PLoS Biol 3(2); Microbes on MacConkeys, T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.com ________ _____ in Cell Count From Binary Fission Generation Cell Number Count 0 1 1 2 2 4 3 8 4 16 5 32 10 1,024 20 1,048,576 Let’s watch a time lapse movie of E. coli population growth ....
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Q How do cells get energy if there is no O 2 available to...

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