12-13_Microbial_Growth___Metabolism_Combo

Q how do cells get energy if there is no o 2

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Q: How do cells get energy if there is no O 2 available to them, or if they can’t use O 2 ? From the Virtual Microbiology Classroom on ScienceProfOnline.com
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______________ When there is no final __________ __________ for the ETC, the electron transport can’t happen. Fermentation is an alternative system that allows glycolysis to continue without the other steps of respiration. Not as energetically efficient as respiration. Produces only 2 ATP . ATP ATP From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Metabolism & Identification of Microbes Some of the specialized media that we have worked with in lab is both selective and differential . The differential properties give us information about bacteria based on its metabolism. Qs: What is the medium in top picture? Is selective …Why? What does it grow? Is differential …Why? What does the differential property reveal about the bacteria growing there? Qs: What is the medium in bottom picture? Is selective …Why? What does it grow? Is differential …Why? What does the differential property reveal about the bacteria growing there ? Images: MacConkey’s Agar (MAC) & Mannitol Salt Agar (MSA), T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Metabolism & Identification of Microbes API-20E The API-20E test is used to ID Gram- negative enteric bacilli-shaped bacteria from the family. System of 20 individual, miniaturized tests used to determine the ____________ ____________ of the organism. Some microbes can metabolize certain molecules while others can’t. When molecules are metabolized, specific waste products are created. From identification of metabolic capabilities, we can zero in on identification of genus and species. Images: API- 20E with results sheet, T. Port; API - 20E results for different species of bacteria, Dr. Charles Gerba , University of Arizona From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Microbial Growth Refers to increase in the ____________ of microbes (reproduction) rather than an increase in ____ of the microbe. Result of microbial growth is the ________ = aggregation of cells arising from single parent cell. The time required for growth and reproduction is known as the doubling or ___________ ______. Image: Glowing Colony E. coli from "Aging and Death in E. coli" (2005) PLoS Biol 3(2); Microbes on MacConkeys, T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.com
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________ _____ in Cell Count From Binary Fission Generation Cell Number Count 0 1 1 2 2 4 3 8 4 16 5 32 10 1,024 20 1,048,576 Let’s watch a time lapse movie of E. coli population growth . From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Bacterial Population Growth Curve Image: Bacterial growth phases , Michal Komorniczak From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Generation Time Under Optimal Conditions (at 37 o C) Organism Generation Time Bacillus cereus 28 min Escherichia coli 12.5 min Staphylococcus aureus (causes many types of infections) 27-30 min Mycobacterium tuberculosis (agent of Tuberculosis) 18 – 24 hrs Treponema pallidum (agent of Syphilis) 30 hrs Images: B. cereus, E. coli
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