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Wild type retrieving sequences matching origins

  • University of Iowa
  • BIO 3110
  • Test Prep
  • KidHackerApe1410
  • 11
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Wild type: Retrieving sequences matching origins placed 10 Kb for the telomeresNothing retrieved in Pot1 mutantsControls:Negative control: No antibodyNegative control: Mutants strain for pot-1If you put the wrong technique, but the rest was right you could get up to 3 pts
Part 2- Question 1(14 pt)You isolated an L1-like transposable element. This transposable element has a transposition mechanismthat is very similar, yet not identical to L1. You have found that the favorable integration site for thistransposable element is in the immunoglobulin gene cluster, at recombination signal sequences (RSSs) ofV(D)J.For simplicity:1)In this system when an enzyme cuts, 100% of the molecule is cut, no residual uncut2)The DNA run on a gel does not contain protein bound to itPanel A is a cartoon of DNA substrate used for the experiment (top). This DNA contains one RSS, a cutsite for REG1/2, located at the center of the molecule and it is end labeled on one side as indicated by thecircle. There are 3 steps (arrows), with 2 intermediate molecules (question marks) that eventually lead tothe product that is indicated on the bottom of the cartoon. You know that the L1-like element integratedwith poly A/T tracks present always in the orientation indicated in the cartoon. The nucleotide sequence ofthe L1-like element is much larger than the DNA substrate.The substrate DNA was incubated with protein extract from cells that: expresses REG1+2 (#2) or L-1 likeelement (presence of L1 DNA that is making all RNA and protein essential for its function, inthepresence of appropriately processed integration site #3), both (#4) or none (#1). The diagrams in B-Crepresent all gels run (up to down) and exposed to film to detect the presence of the end labeled fragment.
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/Human-Heredity-Principles-and-Issues-11th-Edition-9781305251052-1185/
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Chapter 9 / Exercise 7
Human Heredity: Principles and Issues
Cummings
Expert Verified
02:171 Fall 2013Name______________________Examination 3December 15tha)Explain the results of lane 2B
b)Explain the results of lane 4B
c)Explain the results of lane 4B compared to lane 4C and 2C
d)Explain the results of lane 4D compared to lane 4B, and lane 4E compared to lane 4C
02:171 Fall 2013Name______________________Examination 3December 15thIn the following experiment the end labeling was on the other side of the molecule, as in cartoon A(Questions e, f and g are related to this cartoon, but use the information you got from the other parts of thequestion as well)e)Explain the results of lane 4B compared to lane 3B
f)Explain the results of lane 2B compared to lane 1B
g)Fill in the missing steps in diagram C with cartoons representing the DNA and/or DNA/RNAstructure. Indicate ATP, L1-like and REG1+2 next to the appropriate arrowsI gave all the points if there was one mistake, 1 pt if there were 2-3 mistakes, none if there weremore
02:171 Fall 2013Name______________________Examination 3December 15th
02:171 Fall 2013Name______________________Examination 3December 15th
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Human Heredity: Principles and Issues
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Chapter 9 / Exercise 7
Human Heredity: Principles and Issues
Cummings
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