10x 100 1000 focusing the microscope 1 plug in the

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10x * 100 = 1000 Focusing the Microscope 1. Plug in the microscope and turn on the light source. 2. Start with the low power objective in place and the stage all the way down. i. You should start this way every time you change your slide. Created by Diane Gibson
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3. Secure the slide on the stage so that it covers the hole and is in place with the stage clips hugging the slide into the L- shaped metal piece. 4. Looking through the oculars, slowly raise the stage using the course adjustment knob until you begin to see the specimen. i. You may need to adjust the width between oculars or the amount of light coming through the diaphragm. ii. You may also need to slowly move the slide around to find the specimen if you don’t see anything after moving the course adjustment knob. 5. Once you see the specimen, slowly adjust the fine adjustment knob to focus. 6. Record your results and move to the next objective lens. 7. Continue using the fine adjustment knob to focus the specimen, record your results, move to the next objective. 8. If using the oil immersion lens you must apply a teeny tiny drop of oil to the slide cover. Created by Diane Gibson
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Slide Numero Uno 1. Get a piece of newsprint from me that has a lowercase letter ‘e’. 2. Place the newsprint on a slide (from metal slot container). 3. Put a slide cover on top of the newsprint. 4. Once you have the image focused under low power, move the slide to your left. Which way does the image move when viewed through the ocular? right 5. Which way does the image move when you move it to your right? left Changes in Image In the space provided, draw the letter “e” exactly as you see it through the ocular under low power. What three things are different between the specimen on your stage and the image you see through the ocular. 1. backwards 2. larger 3. upside down Field of View The amount of area of a slide that can be seen when viewed through the oculars. 1. Draw the letter “e” as you see it, starting with low power. Remember to follow the focusing procedures previously learned. Record the total magnification next to “X” Created by: Diane Gibson Place slide on the stage in “reading position” e Low Power 40 X (Total Magnification) High Power ___400_X Medium Power _10_X
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  • Fall '19
  • low power, Diane Gibson

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