Benedicts reagent and the amylase reaction mixture

  • Monash University
  • BIO 1011
  • Lab Report
  • JarudeSandstorm
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Benedict’s reagent and the amylase reaction mixture that cuprous oxide precipitate was present which indicates the presence of maltose, whereas in the control tube that contained buffer and starch solution, no cuprous oxide precipitate was present. Table 1. Amylase activity in different seeds Discussion The results show that the three day germinant has the highest amount of mean activity with the 7 day seedling having a little under half the amount of activity
with the 0 day dormant seed having no activity. The dormant seeds do not have any activity, because as the seedling grows, by the time the endosperm is completely used up, the seedling should have reached a stage where its energy and carbon requirements can be met by photosynthesis. In the older seedlings, carbon is obtained through photosynthesis and not through the breakdown of starch. It is seen that the 7 day old seedlings hydrolyse the starch at a slower rate than the 3 day old Germinant seeds. This is due to the fact that there is less endosperm left to be broken down, so less amylase is present. The plant now relies on photosynthesis for its energy needs as its first leaves have developed. It is not assumed that the enzyme becomes less efficient, as the conditions that were tested under were the same, there was just less of it. From the other small experiment conducted involving Benedict’s reagent the precipitate was not formed in the control due to the presence of starch whereas in the experimental tube the precipitate can be seen which indicates the presence of maltose which was predicted in the hypothesis .

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