The presence of a band in lane g and the lack of a

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lanes, the results matched the predictions (Table 1). The presence of a band in lane G and the lack of a band in lane F, indicates that the YMP24 strain is the met2 mutant. DNA amplification occurred with a MET2 Primer A and KAN Primer B, but not with a MET17 Primer A and KAN Primer B. MET2 Primer A and MET17 Primer A are gene-specific primers, but only MET2 Primer A with KAN Primer B allowed exponential, semi-conservative DNA synthesis in the PCR. The presence of a band in lane E, and lack of a band in lane D, indicates that YMP12 strain is the met3 mutant. Only the MET3
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Primer A with KAN Primer B could bind to both native and recombinant chromosomes of met3 to allow exponential, semi-conservative DNA replication. Presence of a band in lane C and lack of a band in lane B indicates that the YMP19 strain has the met17 mutation. Only the MET17 Primer A could bind to a met17 native chromosome and allow DNA synthesis of both native and recombinant met17 chromosomes with KAN Primer B. The molecular weight in lane A, and the band in lane C were faint, while bands in lane E and G were relatively clear. The uneven pronunciation of band illumination suggests an uneven dye distribution, which may have resulted in a false negative for lanes B, D, and F. If a band were visible in lane B, D, or F, the hypothesis that bands did not form because of the forward primer’s inability to bind to genomic DNA would have to be altered. Results
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