This is why the gram stain question is so important

Info icon This preview shows pages 23–26. Sign up to view the full content.

View Full Document Right Arrow Icon
This is why the Gram Stain Question is so Important. It determines which Biochemical Testing Pathway you’ll take. At this Point your Clan -- or some other Clan in your Lab -- has isolated Strains of all but one of these Bacteria (Streptococcus lactis ). ______________________________ “Wear your jewels to bed, Princess?” ‘Yes... and nothing else. Shock you?’ “Nothing shocks me. I'm a scientist.” © 1984 LucasFilm, Ltd.
Image of page 23

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Lab 6 Page 24 The Biochemical Tests are kind of like asking your Lab Partner if they have a Pet with Fur or a Pet with Feathers; then asking if their Pet is an Herbivore or a Carnivore. Just those two Questions could distinguish four very different Pets: a Dwarf Rabbit, a Siamese Cat, a Canary and an Owl: • Does it have Fur or does it have Feathers? (It has Feathers) • Is it an Herbivore or is it a Carnivore? (It’s a Carnivore) Sometimes we’ll ask an additional Question to really nail-down an ID: • Does it reliably deliver Mail to Hogwarts? (Sometimes) • OK, it must be Errol. Our Lab Standards are : Gram Positive Lab Standards • Bacillus cereus • Micrococcus luteus • Staphylococcus epidermidis • Streptococcus lactis Gram Negative Lab Standards • Enterobacter aerogenes • E. coli • Pseudomonas putida In Today’s Lab you’ll be performing (or setting-up) Biochemical Tests on your Clan’s Isolates. We’ll have Lab Standards for the Biochemical Tests you perform Today and for the Biochemical Tests you’ll set-up Today but “read” in the next Lab. This is how Clinical Labs identify Specimens from Patients, or how Environmental Labs identify Specimens from Environmental Samples. __________________________________________________________ You know what your problem is, Princess? You're too used to getting your own way.” ‘And you're too proud to admit that you're crazy about me, Dr. Jones!’ “If you want me Willie, you know where to find me.” ‘Five minutes. You'll be back over here in five minutes.’ “I'll be asleep in five minutes.” ‘Five. You know it, and I know it.’ © 1984 LucasFilm, Ltd.
Image of page 24
Lab 6 Page 25 The Biochemical Tests Gram Positive Biochemical Tests The Game Plan: Your Gram Stain will tell you if you have Gram Positive Rods (Bacillus cereus ) or Gram Positive Cocci (Streptococcus lactis , Micrococcus luteus or Staphylococcus epidermidis ). You’ll do a Catalase Test to determine whether your Bacterium is a Streptococcus lactic (Negative) or a Micrococcus luteus or a Staphylococcus epidermidis (both Positive). Then you’ll streak a YGC Plate to distinguish Micrococcus luteus from Staphylococcus epidermidis . All of these Biochemical Tests are detailed in your Atlas for Microbiology. The Page Number References are for that Text. Gram Stain Morphology “Are you a Rod-Shaped or a Spherical-Shaped Bacterium?” If you have Gram Positive Rods, you have Bacillus cereus . You could confirm this by doing a Spore Stain to show characteristic Endospores (Spores).
Image of page 25

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 26
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern