100%(4)4 out of 4 people found this document helpful
This preview shows page 3 - 5 out of 6 pages.
(e.)Report in the table below for each of the objectives (4x 10x, 40x, & 100x), the corresponding N.A, total magnification, and the calculated theoretical resolution using Abbe’s equation (show your work, indicate the wavelength of light chosen). Discuss the relationship of resolving power to numerical aperture.(f.)Based upon the steps you performed to determine the size of the Paramecium specimen using the ocular reticle and the stage micrometer, list a set of brief instructions to determine the actualsize (in SI units) of any object. Report the calibration of reticle units you determined for each objective in the table below as well. (f.i.)XXPage 3of 6
EXERCISE 1Page 4of 6(2)INDIVIDUAL:Paramecia(permanent dry and fresh wet mounts)(g.)Based on your experience in this exercise, report in tabular form at least two advantages and two disadvantages of permanent dry mounts compared with wet mounts for determining features of specimens as observed by brightfield microscopy. Advantages / DisadvantagesWet mountsDry mountsDisadvantageDifficult to find the movingparamecium Paramecium were inclumps and made itchallenging to see theborders of where onestarted and ended Disadvantage Measuring paramecium wasdifficultDifficult to know thecharacteristicsotherthan size and shape AdvantageParamecium was alive andthe characteristics were moreaccurate Easier to measure lengthand width with a stillparamecium Advantage Paramecium were alone andmade for a good subject toobserveParamecium was easy tofind(h.)Include a digital image of Parameciapresent in a permanent dry as well as a fresh wet mount.