e Report in the table below for each of the objectives 4x 10x 40x 100x the

E report in the table below for each of the

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(e.)Report in the table below for each of the objectives (4x 10x, 40x, & 100x), the corresponding N.A, total magnification, and the calculated theoretical resolution using Abbe’s equation (show your work, indicate the wavelength of light chosen). Discuss the relationship of resolving power to numerical aperture. (f.)Based upon the steps you performed to determine the size of the Paramecium specimen using the ocular reticle and the stage micrometer, list a set of brief instructions to determine the actualsize (in SI units) of any object. Report the calibration of reticle units you determined for each objective in the table below as well. (f.i.)   X X Page 3 of 6
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EXERCISE 1 Page 4 of 6 (2) I NDIVIDUAL : Paramecia (permanent dry and fresh wet mounts) (g.) Based on your experience in this exercise, report in tabular form at least two advantages and two disadvantages of permanent dry mounts compared with wet mounts for determining features of specimens as observed by brightfield microscopy. Advantages / Disadvantages Wet mounts Dry mounts Disadvantage Difficult to find the moving paramecium Paramecium were in clumps and made it challenging to see the borders of where one started and ended Disadvantage Measuring paramecium was difficult Difficult to know the characteristics other than size and shape Advantage Paramecium was alive and the characteristics were more accurate Easier to measure length and width with a still paramecium Advantage Paramecium were alone and made for a good subject to observe Paramecium was easy to find (h.) Include a digital image of Paramecia present in a permanent dry as well as a fresh wet mount.
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  • Spring '10
  • ELLEN
  • Height, Statistical hypothesis testing, Statistical significance, Elodea

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