E report in the table below for each of the

(e.)
Report in the table below for each of the objectives (4x 10x, 40x, & 100x), the corresponding
N.A, total magnification, and the calculated theoretical resolution using Abbe’s equation
(show your work, indicate the wavelength of light chosen). Discuss the relationship of
resolving power to numerical aperture.
(f.)
Based upon the steps you performed to determine the size of the Paramecium specimen using
the ocular reticle and the stage micrometer, list a set of brief instructions to determine the actual
size (in SI units) of any object. Report the calibration of reticle units you determined for each
objective in the table below as well.
(f.i.)
X X
Page
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EXERCISE 1
Page
4
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6
(2)
I
NDIVIDUAL
: Paramecia (permanent dry and fresh wet mounts)
(g.)
Based on your experience in this exercise, report in tabular form at least two advantages and
two disadvantages of permanent dry mounts compared with wet mounts for determining
features of specimens as observed by brightfield microscopy.
Advantages / Disadvantages Wet mounts Dry mounts
Disadvantage Difficult to find the moving
paramecium
Paramecium were in
clumps and made it
challenging to see the
borders of where one
started and ended
Disadvantage Measuring paramecium was
difficult
Difficult to know the
characteristics other
than size and shape
Advantage Paramecium was alive and
the characteristics were more
accurate
Easier to measure length
and width with a still
paramecium
Advantage Paramecium were alone and
made for a good subject to
observe
Paramecium was easy to
find
(h.)
Include a digital image of Paramecia present in a permanent dry as well as a fresh wet mount.