28 mm 20 mm 19 mm r3 21 mm 5 mm 25 mm 5 mm 22 mm 17

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28 mm 20 mm 19 mm R3 21 mm 5 mm 25 mm 5 mm 22 mm 17 mm Y5 21 mm 18 mm 28 mm 25 mm 20 mm 19 mm G3 20 mm 19 mm 34 mm 33 mm 19 mm 18 mm R3+Y5 21 mm 20 mm 35 mm 29 mm 21 mm 20 mm B1+G3 23 mm 22 mm 35 mm 30 mm 21 mm 20 mm B2+R3 22 mm 21 mm 31 mm 8 mm 20 mm 19 mm Table 2: Measurements for the known and unknown dyes with NaCl (0.1%). Dyes NaCl (0.1%) Solvent: Spot: B1 17 mm 16 mm B2 9 mm 7 mm R3 18 mm 6 mm Y5 18 mm 15 mm G3 20 mm 16 mm Unknown I 19 mm 17 mm Unknown E 22 mm 21 mm Unknown C 22 mm 17 mm Results Sample calculation of the retention factor of B1 in water:
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MICROFLUIDIC PAPER CHROMATOGRAPHY 3 B 1: Rf = dspot dsolvent = 20 23 = 0.87 Table 3: Retention Factors Dyes Water NaCl (0.1%) Propanol (20%) B1 0.87 0.97 0.94 B2 0.96 0.97 0.95 R3 0.24 0.20 0.77 Y5 0.86 0.89 0.95 G3 0.95 0.97 0.95 R3+Y5 0.95 0.83 0.95 B1+G3 0.96 0.86 0.95 B2+R3 0.95 0.26 0.95 Table 4: Retention factors for known and unknown dyes in NaCl. Dyes Retention Factors in NaCl B1 0.94 B2 0.77 R3 0.33 Y5 0.83 G3 0.80 Unknown I 0.89 Unknown E 0.95 Unknown C 0.77 Discussion Questions: (a). If the spot is larger, the spot will spend more time to reach the same height than the smaller spot would. (b). Yes, the size of spot depends on the mobile phase. It is transported faster when it is smaller. (c). The size of spot is larger with low polarity solvent. The size of spot is smaller with high polarity solvent.
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