Lab__3a_Isolation_Streak_Plate_Method

Dont scrape so hard that you have pieces of agar in

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bacteria is growing as a thin film on top of the media! Don’t scrape so hard that you have pieces of agar in your sample! From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: E. col i growing on TSY agar in slant tube and in Petri dish, T. Port If obtaining bacterial sample from slant tubes: - never pick up test tube by the cap. - do NOT set cap down on lab bench - flame neck of the test tube before & after obtaining sample.
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Streak plating is used to isolate a single type of bacteria. This technique spreads out original “parent bacteria” in a sparse pattern that ,after growth, results in individual colonies . After incubation, the 4 th quadrant of your plate should have dots. These small “dots” are individual colonies, and represent millions of bacteria of the same type. Isolation Streak Plates & Aseptic Technique * IMPORTANT!!!: Be very gentle when streaking the sample onto the plate. Try not to gouge the surface of the medium with your inoculation loop.
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