1 wv malachite green 001 wv methyl violet 02 wv picric acid 001 wv pyronin B 1

1 wv malachite green 001 wv methyl violet 02 wv

This preview shows page 95 - 97 out of 594 pages.

of hydroxylamine, 0.1% (w/v) malachite green, 0.01% (w/v) methyl violet, 0.2% (w/v) picric acid, 0.01% (w/v) pyronin B, 1% (w/v) sodium deoxycholate and 0.1% (w/v) sodium nitrate, and variably in 0.01% (w/v) chlorophenol red and 20 |ig/ml of sodium azide. Acid phosphatase, aryl sulphatase and catalase are produced, but not acetamidase, benzamidase, isonicotinamidase, nicotinamidase, pyrazinamidase or succinimidase. /?-aminobenzoate, /^-aminosalicylate, sodium salicylate and urea are attacked, but not allantoin, sodium hippurate or Tweens. Acid is produced from glucose and mannose, but not arabinose, dulcitol, fructose, galactose, inositol, mannitol, rhamnose, sorbitol, sucrose, trehalose or xylose. Neither sodium benzoate, sodium malonate nor sodium oxalate are utilised as the sole source of carbon, but variable reponses may be recorded with sodium citrate, sodium fumarate and sodium succinate. L-serine and sodium L-glutamate but not acetamide, benzamide, glucosamine hydrochloride or nicotinamide are utilised as the sole source of carbon and nitrogen. Mycolic acids are present. The G + C ratio of the DNA is in the range of 61-65 mol %; with an average value of 63zb 1.7 mol % (Arakawa and Fryer, 1984). The fish isolates were considered to be related to Myc. chelonei, although somewhat distinct from the current subspecies, i.e. chelonei and abscessus. For example, the fish isolates were unable to grow at 37°C, produce nicotinamidase or pyrazinamidase and degrade sodium hippurate or produce acid from trehalose, in contrast to the two validly published subspecies. However, there was an overall phenotypic similarity in excess of 85%. Thus, from this similarity together with the results of mycolic acid determination (the fish isolates were identical with Myc. chelonei subsp. chelonei and Myc. chelonei subsp. abscessus when examined by two-dimensional thin-layer chromatography of acid methanolysates), it was proposed to establish a new sub- species, i.e. Myc. chelonei subsp. piscarium. Nevertheless, to date this subspecies has not been formally proposed in the refereed scientific literature. Myc. chelonae is slowly increasing in significance in farmed fish. For example, Myc. chelonae isolates, not allocated to subspecies, were recovered from diseased Atlantic salmon in two farms in Shetland (Bruno et al., 1998).
Image of page 95
72 Bacterial Fish Pathogens Myc. gordonae was described as comprising acid-fast rod-shaped cells, which was identified presumptively by 16S rRNA sequencing (Sakai et ai, 2005). Myc. montefiorense was regarded as a cause of granulomatous skin lesions in moray eels (Levi et al, 2003). Mycobacterium montefiorense Cultures comprise slow-growing (20 weeks at 25°C), non-chromogenic acid-fast coccobacilli (on blood agar) or rods (on Middelbrook agar), which do not produce catalase or arylsulphatase, do not reduce nitrate, degrade Tween 80 or urea, or grow at > 30°C (growth occurred at 25°C) or in 5% (w/v) NaCl (Levi et ciL, 2003).
Image of page 96
Image of page 97

You've reached the end of your free preview.

Want to read all 594 pages?

  • Spring '20
  • Bacteria, representative, gram-negative bacteria

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern

Stuck? We have tutors online 24/7 who can help you get unstuck.
A+ icon
Ask Expert Tutors You can ask You can ask You can ask (will expire )
Answers in as fast as 15 minutes
A+ icon
Ask Expert Tutors