Single phosphorylation sites in Acc1 and Acc2 regulate lipid homeostasis and the insulin–sensitizing

Xenobiotica the fate of foreign compounds in

This preview shows page 11 - 13 out of 16 pages.

Xenobiotica; the fate of foreign compounds in biological systems. 1994; 24:49–57. [PubMed: 8165821] 36. Clark JM, Brancati FL, Diehl AM. Nonalcoholic fatty liver disease. Gastroenterology. 2002; 122:1649–1657. [PubMed: 12016429] 37. He L, et al. Metformin and insulin suppress hepatic gluconeogenesis through phosphorylation of CREB binding protein. Cell. 2009; 137:635–646. [PubMed: 19450513] 38. Turban S, et al. Defining the contribution of AMP-activated protein kinase (AMPK) and protein kinase C (PKC) in regulation of glucose uptake by metformin in skeletal muscle cells. The Journal of biological chemistry. 2012; 287:20088–20099. [PubMed: 22511782] 39. Dzamko N, et al. AMPK beta1 deletion reduces appetite, preventing obesity and hepatic insulin resistance. The Journal of biological chemistry. 2010; 285:115–122. [PubMed: 19892703] Fullerton et al. Page 11 Nat Med . Author manuscript; available in PMC 2016 July 28. CIHR Author Manuscript CIHR Author Manuscript CIHR Author Manuscript
Image of page 11

Subscribe to view the full document.

40. Steinberg GR, et al. Whole body deletion of AMP-activated protein kinase {beta}2 reduces muscle AMPK activity and exercise capacity. The Journal of biological chemistry. 2010; 285:37198– 37209. [PubMed: 20855892] 41. Ussher JR, et al. Stimulation of glucose oxidation protects against acute myocardial infarction and reperfusion injury. Cardiovascular research. 2012; 94:359–369. [PubMed: 22436846] 42. Galic S, et al. Hematopoietic AMPK beta1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity. J Clin Invest. 2011; 121:4903–4915. [PubMed: 22080866] 43. Schertzer JD, et al. NOD1 activators link innate immunity to insulin resistance. Diabetes. 2011; 60:2206–2215. [PubMed: 21715553] 44. Steele R. Influences of glucose loading and of injected insulin on hepatic glucose output. Annals of the New York Academy of Sciences. 1959; 82:420–430. [PubMed: 13833973] 45. Preiss J, et al. Quantitative measurement of sn-1,2-diacylglycerols present in platelets, hepatocytes, and ras- and sis-transformed normal rat kidney cells. The Journal of biological chemistry. 1986; 261:8597–8600. [PubMed: 3013856] Fullerton et al. Page 12 Nat Med . Author manuscript; available in PMC 2016 July 28. CIHR Author Manuscript CIHR Author Manuscript CIHR Author Manuscript
Image of page 12
Fig. 1. Acc1 Ser79 and Acc2 Ser212 are essential for inhibiting enzyme activity and regulating liver fatty acid metabolism ( a ) Representative Western blot of Ampk– α Thr172, zcc1 Ser79 (bottom band) and Acc2 Ser212 (top band) phosphorylation in liver of WT, Acc1KI and Acc2KI and AccDKI mice. ( b ) Acc1 and ( c ) Acc2 activity with and without citrate (10 mM) in WT and AccDKI liver ( n = 5 WT and 6 DKI). ( d ) Liver malonyl–CoA in the fed–state ( n = 8). ( e ) The incorporation of [ 3 H]–acetate into TAG as a measure of de novo lipogenesis and ( f ) [ 14 C]–palmitate oxidation in primary hepatocytes ( n = 3, from at least 3 separate experiments). ( g ) Total adiposity in chow-fed WT and AccDKI ( n = 10 WT and 14 DKI). ( h ) Liver DAG and TAG ( n = 6 WT and 8 DKI). ( i ) Histological representation (scale bar is 100 μm) and quantification of collagen staining in liver sections ( n = 6). ( j ) Activation of liver Pkc– ε as demonstrated by membrane–association ( n = 7). Data are expressed as means ± SEM, * P < 0.05, ** P < 0.01 and *** P < 0.001 relative to WT, as determined by ANOVA and Bonferonni post hoc test or a Student’s
Image of page 13
You've reached the end of this preview.
  • Winter '19
  • Robert S Kiss
  • Insulin resistance, Fatty acid metabolism

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern