The results are shown below where indicates the

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tagged sites (STSs, a class of molecular markers) 1 through 5. The results are shown below, where + indicates the presence of an STS in a particular clone: STS ––––––––––––––––––– DNA 1 2 3 4 5 ––––––––––––––––––––––––––––––––– A + + + B + + C + + D + +
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Test Bank: Chapter Nineteen Draw a contig map of these cloned fragments, showing the relative positions of the STSs: φ 2. A microsatellite probe whose locus shows tight linkage to the locus of the late-onset disorder Huntington disease (HD) was used to analyze the following family. Autoradiograms using the probe are shown: Paternal grandfather Father Mother Child (HD) (HD) (unaffected) (?)         Is the child likely to develop Huntington disease later in life? 4. A pair of PCR primers in unique genomic DNA is designed to amplify a microsatellite region. In a couple, this pair of primers amplifies bands of 1.2 and 1.4 kb in the man, and bands of 0.8 and 1.3 kb in the woman. What offspring genotypes are expected in regard to amplification by these primers, and in what proportions? 5. A minisatellite probe is used to prepare DNA fingerprints of a man and a woman. The man shows five bands of various sizes and the woman seven bands, some the same as and some different from the man’s. One of their children is also tested, and he shows none of his parents’ bands. Is this possible? If so, what is the probability? Explain your answer. 6. Assume that a fragment of human DNA 1 kb in size, when cloned and used as a probe, reveals an RFLP in total DNA cut with the restriction enzyme Eco RI. Individuals in family X show one of the following patterns: (i) one fragment of 6 kb; (ii) two fragments of 4 kb and 2 kb; or (iii) three fragments of 6 kb, 4 kb, and 2 kb. A late-onset autosomal dominant disease also occurs in this family. All the people with the disease show pattern (iii). Unaffected family members show pattern (ii).
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