We must be cautious 1977 lucasfilm ltd lab 1 page 18

Info icon This preview shows pages 17–20. Sign up to view the full content.

View Full Document Right Arrow Icon
We must be cautious. © 1977 Lucasfilm, Ltd.
Image of page 17

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Lab 1 Page 18 Escherichia coli (E. coli ) [Atlas Page 144] E. coli are also Gram Negative, Facultative Aerobic, Non-Spore-Forming Rods, but all E. coli are Symbiotic and are found only inside the Enteric Tract of Warm- Blooded Animals (Birds or Mammals). Until recently E. coli was considered a Non-Pathogenic Species. But in 1993 an Outbreak of Food-Borne Infection involving E. coli killed a lot People in Washington and Idaho. It seems that certain Strains of E. coli had Netflixed the Pathogenicity Island DVD and had crossed-over to The Dark Side. When grown on EMB Medium E. coli produces Purple Colonies and a characteristic Fly-Butt Green Metallic Sheen on the Surface of the Medium. E. coli is one of the most intensively studied of all Species. It has been the Lab Rat for Classical Experiments in Microbiology and Molecular Biology. All Escherichia in Nature are entirely Symbiotic, occurring in large Numbers in the Gut of Warm-Blooded Animals. Escherichia is not normally Pathogenic, but can cause serious Infections when it gains Access to the Bladder. E. coli can also cause Peritonitis if the Intestine is punctured -- say by a Gun or Knife Wound -- and the Intestinal Contents are released into the Peritoneal Cavity. You should treat Escherichia as a Pathogen. E. coli is on EMB Medium. The Production of buttloads of Acid results in the Precipitation of the metachromatic Dye Methylene Blue, making the Colonies appear dark Purple and the Agar to take-on a Fly-Butt Green Metallic Sheen. When grown on Nutrient Agar, E. coli Colonies are Clear-to-White and indistinguishable from Enterobacter aerogenes Colonies. The IMViC Test is also used to distinguish E. coli from Enterobacter . The IMViC Test consists of the combined Results of four Biochemical Tests: Indole, Methyl Red, Voges-Proskauer and Citrate (the ”i” was inserted to supply a Vowel). The Results from IMViCs for E. coli and Enterobacter are neatly Reciprocal: Indole MR VP Citrate E. coli + + - - Enterobacter - - + + ____________________________ “You don't need to see his identification.” ‘We don't need to see his identification.’ “These aren't the droids you're looking for.” ‘These aren't the droids we're looking for.’ “He can go about his business.” ‘You can go about your business.’ “Move along.” ‘Move along... move along.’ © 1977 Lucasfilm, Ltd.
Image of page 18
Lab 1 Page 19 Background for Lab 1 Exercises Aseptic Technique Sterile Technique Aseptic Technique ( G= without rot) is critically Important! Sterile Technique (G= barren) is critically Important! Aseptic Technique is the sine qua non (Latin for “without this nothing”) of Microbiology. The Techniques you’ll start learning Today are absolutely Essential for all of our Exercises and for identifying your Unknown Cultures. The basic Idea is to use Sterile or sterilized Instruments and to minimize the Time a Serological Pipette, Test Tube or Petri Plate is uncovered. We want to move our Bacteria from Container to Container without letting any other Bacteria into these Containers and without letting our Bacteria out of these Containers.
Image of page 19

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 20
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern