The gene contains three exons two introns a promoter

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J. The gene contains three exons, two introns, a promoter, and a site in I for poly(A) addition. (a) What segment or segments of the gene will be represented in the initial RNA transcript? List the appropriate letter or letters. (b) What segment or segments of the gene will be found in the completely processed transcript? (c) What segment or segments of the gene in the processed transcript will have additional nucleotides added to them? (d) What segment or segments of the gene contain the CCAAT box and TATA box elements? (e) What segment or segments of the gene will possess the translation initiation codon? (f) In a large sample of null alleles, would mutant sites be expected in segments C and D, at the boundary of G–H, or both? If mutant sites exist, would the transcript be detected? Would a polypeptide chain be observed?
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Evolutionary Genetics 3.In the silkmoth, a single gene encodes the protein that is the major constituent of the cocoon. (a) The mRNA encoding this protein was isolated from silkmoth larvae and hybridized with silkmoth DNA. The following electron micrograph was obtained: (1) Explain the loops in the DNA strand. (2) Show on the diagram which side of the mRNA is its 5´ and which is its 3´ end. Explain. (b) A mutant silkmoth was isolated that cannot make the cocoon protein. The mutant makes mRNA encoding the protein at the same concentration as the wild type, but the mRNA molecules are slightly longer than usual. In addition, an electron micrograph of the mRNA-DNA hybridization with mRNA from the mutant organism shows the following: Suggest a possible explanation for the nature of the mutation. 4. Consider the following piece of messenger RNA: 5´-AUGGAGUCGUUAAUUAAACCGGUGCGGAUCGUAUUUAGUCCCCAC-3´ (a) Draw both strands of the segment of DNA from which this mRNA was transcribed. (b) State which of the DNA strands the RNA synthesizing enzyme used as template for the transcription process. (c) Using the codon chart, give the amino acid sequence of the protein that would be produced by translation of the mRNA, assuming the ribosome moved along the mRNA from left to right.
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