And one plate of syta the various plates will be

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and one plate of SYTA. The various plates will be inoculated, incubated, and examined as described below and summarized in Tables 1-1 and 1-2. Table 1-1. INOCULATION CHART Inoculate the following as instructed in lab manual Source of Inoculum MEDIA Blood SYTA YTA YTA + cyclo Sab Sab + cyclo Skin swab 2 - - - - - Tongue swab - 1 - - - - Air - - 1 1 1 1 Sab = Sabouraud's agar, cyclo = cycloheximide Skin swab plates. Inoculate the two blood agar plates as follows: First, wet the tip of the swab by dipping the tip into a sterile solution of water or saline. Rub the sterile wet swab against the palm of your hand or your fingers and then streak one quadrant of the first plate with the swab. Next, wash your hands well with the soap provided. Again swab the skin on your hand with a second sterile wet swab and streak one quadrant of the second plate with the swab. Complete the streaking on both plates with a loop using the quadrant method: Incubate the plates at 37ºC for 24 hrs. Streptococcus salivarius (tongue). Each student will be provided with one SYTA plate. Inoculate this plate by rubbing a sterile swab on your tongue. Note your tongue should be moist.
Page 5 of 7 Pass the swab across the SYTA plate without digging the agar. Discard the contaminated swab in the red biohazard sharps box.Complete the streaking by the quadrant method with a loop. Incubate the plate at 35 ºC for 48 hrs. Table 1-2. INCUBATION CHART Incubate the cultures as follows: INOCULUM NUMBER OF PLATES TEMPERATURE TIME Skin swab 2 35ºC 24 h Tongue swab 1 35ºC 48 h Air 4 Ambient 7 days Second Period . Examine blood plates in the biosafety hood . Note differences, if any, in colony types and abundance of growth on the plates incubated at different temperatures and on the plates from the skin swabs. Note changes in the blood in the vicinity of some colonies. Discard your blood agar plates at the end of the lab period in the red biohazard bins . Examine SYTA plates from tongue swab and look for translucent, gummy colonies, typical of Streptococcus salivarius on a sucrose medium. Large, white colonies may also be observed as well as numerous small ones. The big ones may be yeast, and this distinction should be confirmed by microscopic examination. If you find evidence of S. salivarius , try to obtain a sample from a well isolated colony for wet mount examination and do a streak plate on GYC (contains glucose). Incubate plates at 37ºC for 3-5 days. SAVE your SYTA plate for comparison of colonies from GYC plate. Air: Please tape these four plates together and take them around campus. When you have decided on the location to take your air samples, remove the covers from the dishes of YTA and Sabouraud's agar (both ± cycloheximide) and expose the contents at the same location to the air for 10 minutes. Replace covers, tape them together and return the plates to your T.A.

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