E.coli, P Aeruginosa, E. Faecalis, and S. Aureus. Four sections of each plate were inoculated. Then 4
Usman Syed Microbiology Lab2/17/2019 TGY broth tubes and 4 synthetic media broth tubes that were created in part 1 were inoculated. This was done to see if different media would promote or inhibit the growth of the bacteria. Lastly, students isolated S. Aureusinto a single colony by using the pure culture technique. Each student transferred a loop of the culture from the slant onto the agar plate. A streak was then performed in long straight lines with at least four turns for four different quadrants. At each turn the loop was flamed, and the bacteria was deposited onto the next quadrant.Results and DiscussionWhen observing for sterility, the tubes placed in the autoclave had no bacterial growth. The appeared clear and translucent. In the non-autoclave tubes, bacteria grew. The complex medium tubes had sediment growth while the synthetic tubes had pellicle growth. It can be concluded that those tubes that were placed in the autoclave machine had no bacterial growth and had no contamination. When observing for microorganism growth on the different culture media, all bacteria in the synthetic medium had no growth. However, bacteria placed in the complex TGY medium had growth.E.coliand P. Aeruginosahad sediment growth and appeared cloudy with some turbidity. E. Faecalisand S. Aureus had turbidity and also had white sediment growth on the bottom. It could be concluded that the synthetic medium could not support the growth of the bacterial colonies. In the MacConkey plate, only E. coliand P. aeruginosagrew. E colihad pinkish growth however it was difficult to differentiate the morphology of the bacterial colony on the plate. P.
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