Utilizing the albumin reagent set method resulted in

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diagnose disorders, disease, and other abnormalities. Utilizing the Albumin Reagent Set method resulted in a color conformation which was due to albumin binding with bromocresol green (BCG) to yield a blue- green color complex. Principles: When albumin binds with BCG dye, a blue-green color complex is yielded. This is measured at 630 nm, and the color increase is proportional to the albumin concentration present in the sample. Materials and Methods: The equipment used during the laboratory experiment include a Spectrophotometer, pipettes, automatic pipettes, disposable pipette tips, cuvettes, test tube racks, heat blocks, and timers. The reagents, QCs, and standard used in this experiment include the Patient Level 2- Unassayed Chemistry Control (Human) which expired on June 30, 2010 and had the lot number 16412, the Albumin Reagent which expired in April of 2012 and had a lot number of 009101-173, , the Standard (4.0 mg/dl) which expired in January of 2010 and had a lot number of 800901-211, Quality Control 1 which expired on March 31, 2011 and had a lot number of CHA 11031, and Quality Control 3 which expired on March 31, 2011 and had a lot number of CHA 11033. Results: Sample Absorbance at 630 nm Blank 0.00 Patient 0.975 Standard 0.867 QC Level 1 0.924 QC Level 3 0.698 Calculations: Blank: 0.00/0.867 x 4.0 mg/dl = 0.00 mg/dl Patient: 0.975/0.867 x 4.0 mg/dl = 4.5 mg/dl
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Patterson Standard: 0.867/0.867 x 4.0 mg/dl = 4.0 mg/dl QC Level 1: 0.924/0.867 x 4.0 mg/dl = 4.26 mg/dl
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