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5.How should the agar plates be stored to allow effective bacterial growth overnight?Clicker Questions: 1.What is the purpose of Chelex Beads?2.Why is the enzyme helicase not needed in this reaction?3.Who will have the longest segment of DNA D1S80?4.What is NOT in the Master Mix?a.Taq Polymerase - yesb.dNTPs -yes c.Primers - nod.Human Genomic DNA -noe.Primers and human genomic DNA are not in the Master Mix5.On which plate will tyrosinase oxidize tyrosine
6.On which plate will tyrosinase be produced?Exercise 11 Lecture:●Chelex beadsto bind ions needed by DNase, are a chelator that can interfere with PCR if not removed ●Helicaseis not needed in PCR because mixture is initially heated to 95 degrees Celsius●Who will have the longest segment of DNA for DIS80- 24, 31●On what plate will tyrosinase oxidize tyrosine?○+AMP +Lac +Tyr +Cu2+●Lactose is needed for RNA Polymerase to transcribe tyrosinaseNotes:What was the purpose of the two hot water baths of the buccal cells? heat shock to let in DNA into the competent plasmidsThe buccal cells did NOT needed to be heat shocked. It was placed in a hot water bath to break up the hydrogen bonds between neighboring protein molecules. It was then placed in a hotter hot plate to break up the bonds between protein chains (Now all you have are separated amino acid chains).●PCR: amplifies short segments of DNA and is based on the semiconservative model of replication. ○Vial for PCR typically contains:■the segment of DNA to be copied(template DNA)■enzyme Taq DNA Polymerase: heat-resistant, reads the template strand of DNA from the 3’-5’ end. It then synthesizes the complementary DNA strand from the dNTPS beginning at the primer at the 3’ end of the growing chain in order to extend the DNA ■dNTPS(monomers of DNA): building blocks from which Taq Polymerase synthesizes a complementary strand■primers provided: bind to the template strand which signals for Taq Polymerase to beginadding nucleotides ■buffer solution: provides optimal pH and ionic conditions for DNA to be amplified ■Mg2+cofactor:used to provide a suitable chemical environment for optimum activity andstability of the DNA Polymerase ○