Fermentative metaboHsm Production of Arginine dihydrolase NS Catalase P

Fermentative metabohsm production of arginine

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Fermentative metaboHsm + + Production of: Arginine dihydrolase NS Catalase + + P-galactosidase NS H2S + + Indole + + Lysine decarboxylase + NS Ornithine decarboxylase + NS Oxidase - NS Phenylalanine deaminase NS Methyl red test + NS Nitrate reduction + + Voges Proskauer reaction Degradation of: AescuHn, casein, gelatin Tributyrin, urea NS DNA, elastin, lecithin Tween 20, 40, 60 and 80 - - Chitin Blood + + UtiHsation of: Sodium citrate, sodium malonate NS Growth at: 42° C + + 3% (w/v) sodium chloride + + 8% (w/v) sodium chloride Acid production from: Adonitol, aescuHn, erythritol NS Arabinose, cellobiose, dulcitol Fructose, galactose, glycerol + NS Glucose, maltose + + Inositol - NS Lactose, mannitol, raffinose Mannose + NS Rhamnose, salicin, sorbitol Sucrose, trehalose, xylose ^ From Sakazaki and Tamura (1975), Wakabayashi and Egusa (1973), Amandi et al. (1982) and Nakatsugawa (1983). b From Hoshina (1962) NS = Not stated
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Characteristics of the pathogens: Gram-negative bacteria 105 DNA, gelatin, starch or urea, reduce nitrates, utilise malonate, and produce acid from arabinose, glucose, lactose, maltose, mannitol, melibiose, rhamnose, salicin, trehalose and xylose, but not adonitol, cellobiose, dulcitol, glycerol, inositol, sorbitol or sucrose. The methyl red test is positive, but the Voges Proskauer reaction is negative. Growth occurs at 37°C, but not at 5 or 42°C. The organisms coincided with the description of Esch. vulneris (Brenner et al, 1982). Hafnia alvei In 1988, an apparently new Brucella-likQ bacterium was described as a pathogen of rainbow trout in Bulgaria (Gelev and Gelev, 1988). Although antigenic relationships to Brucella abortus were reported, the organism displayed marked similarities to the Enterobacteriaceae. Subsequent investigation led to a realisation that the organism was, in fact, Hafnia alvei (Gelev et ai, 1990). Subsequently, Haf. alvei was recognised as the cause of mortalities among cherry salmon (O. masou) in Japanese farms (Teshima et ai, 1992). Isolates matched the species description, with the exception of lack of motility and utiHsation of D-tartrate. Hafnia alvei The isolate comprises small coccoid or slightly elongated, motile. Gram-negative rods which produce catalase, P-galactosidase and lysine and ornithine decarboxyl- ase, but not arginine dihydrolase, H2S, indole or oxidase. The methyl red, nitrate reduction and citrate utilisation tests and Voges Proskauer reaction (weakly positive) are positive, but not malonate utilisation or phenylalanine deaminase. Neither aesculin, DNA, gelatin, lipids nor urea is degraded. Acid is produced from arabinose, cellobiose, glucose (but not gas), glycerol, maltose, mannose, mannitol, rhamnose, trehalose and xylose, but not from adonitol, dulcitol, erythritol, lactose, melibiose, raffinose, salicin, sorbitol or sucrose. Growth occurs at 41°C. It was deemed that there was a common antigenic determinant in the LPS with Brucella abortus and Y. ruckeri (Gelev and Gelev, 1988). However, the presence of motility and the negative reaction in the oxidase test precludes a relationship with bona fide Brucella taxa. There was 82-100% re-association between the fish isolates and Haf. alvei
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  • Spring '20
  • Bacteria, representative, gram-negative bacteria

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