Also mutant d is cis acting only from line 4 in table

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Also, Mutant d- is cis -acting only (from Line 4 in Table 2), thus it cannot be a super-repressor or activator.
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(d) Which mutation is most likely to alter the operator for the Sug operon? Answer: Mutant c- In Line 3 of Table 2, Mutant c- is cis to SugA and SugA activity is constitutive. Mutant c- is trans to the wt SugB gene, yet SugB remains uninducible; thus, Mutant c- is not trans -acting. Because Mutant c- is cis-acting only, it likely only affects the operator of the Sug operon. You could make this same conclusion from Line 2, except SugA and SugB are reversed. (e) Which mutation is most likely to inactivate the regulatory protein that controls the Sug operon? Answer: Mutant e- Process of elimination. Also, Line 1 of Table 2 tells us that Mutant e- is “recessive,” so a wt e will restore normal function on the other genome (in this case, that of the F’ plasmid). (f) Is the Sug operon controlled by a repressor or by an activator? Answer: repressor The only mutation that could possibly be an activator is Mutant d-. But since we determined that Mutant d- affects the promoter, we can rule out the possibility of this operon being controlled by an activator (or super-repressor).
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