13 what is the sequence of the dna template strand

13. What is the sequence of the DNA template strand from which this transcript was made? (It’s 5’—
end would begin as…)
A) 5’—GGC…—3’ B) 5’—CAU…—3’ C) 5’—CAT…—3’
D) 5’—CCG…—3’ E) 5’—GAT…—3’
14. Disregarding any Shine-Dalgarno sequence, find the protein-coding message in the mRNA above.
How many amino acids are in the translated peptide.
15. Let us say that the above protein is bacterial andenyl cyclase (it is not, but just pretend), and let us
say that a mutation arises in this gene such that it cannot bind ATP. With reference to the lac operon,
would this cell display beta-galactosidase activity in the presence of glucose and no lactose if it’s lac
operon genotype were: I
s
O
c
Z
+
. Consider both positive and negative control, and assume that all
components must be in place for gene expression to happen efficiently (i.e., an all-or-nothing
response).
16. Answer question 16 above but this time ignore the role of positive control. In other words, with
reference to the lac operon, would this cell display beta-galactosidase activity in the absence of lactose
if it’s lac operon genotype were: I s O c Z + .

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(E) POPULATION & QUANTITATIVE GENETICS
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17 . In a large herd of 5,468 sheep, 76 animals have yellowish wool, and the rest of the herd has white
wool. Yellow wool is inherited as a recessive trait. Assume the herd is in Hardy-Weinberg equilibrium.
What is the frequency of the yellow allele ( q )?
A) 0 < q < 0.2 B) 0.2 < q < 0.4 C) 0.4 < q < 0.6
D) 0.6 < q < 0.8 E) 0.8 < q < 1.0
18. A study of quantitative variation for abdominal bristle number in Drosophila yielded estimates of
V
P
= 6.08, V
G
= 3.17, and V
E
= 2.91. Assuming the interactions between genetics and environment can
be ignored, what is the broad-sense heritability?
19. A fish breeder notes that wild sardines have a mean body length of 18 cm (six-week-old
fingerlings). He has a stock of domesticated sardines that are especially tasty, but they have a mean
length of only 10 cm at 16 weeks. He wishes to increase the rate of growth in his stock by selecting for
increased length at six weeks after hatching. He selects large adult fish from his domesticated stock,
and this pool of breeders at 16-weeks has a mean length of 15 cm. He breeds this group to produce a
new generation of fingerlings, and these progeny have a mean length of 12.5 cm at 16 weeks. The
adults in his stock that were not allowed to breed had a mean length of 7.2 cm. Estimate the narrow-
sense heritability of fingerling length at six weeks of age given these data.

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(F) SUPPLEMENTARY PROBLEMS I – gene regulation
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Refer to the diagram above for questions in this section.