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MIC 101 Exam 1 Winter 2013

What else would confirm your suspicion as to the

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What else would confirm your Suspicion as to the Identity of this Bacterium? A. The Presence of a Yellow-Green Color on F Agar due to Pyoverdin, a fluorescent Siderophore. B. The Presence of Heat-Resistant Endospores that can survive being heated to 80°C for 20 Minutes. C. The Following IMViC Results: Indole MB VP Citrate + + - - MB= Methylene Blue VP= Verdopyin ________________________________________________________ D. The Following IMViC Results: Indole MR VP Citrate - - + + MR= Methyl Red VP= Voges Proskauer
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MIC 101 Exam 1 Page 3 4. While waiting for this Smear to Air Dry, you and your Clan Members begin plotting your Identification Strategy. You know your Unknown Bacterium is one of the Bacteria in the Rogues Gallery in Lab 1. Which of the Following is a correct Assumption about your Unknown Bacterium? A. • If your Unknown is a Soil Isolate, it will be Gram Positive Rods. • If your Unknown is Skin Isolate, it will be Gram Positive Cocci. • If your Unknown is an Enteric Bacterium, it will be Gram Negative Rods. B. • If your Unknown is a Soil Isolate, it will be either Gram Positive Cocci or Gram Negative Rods. • If your Unknown is Skin Isolate, it will be Gram Positive Cocci. • If your Unknown is an Enteric Bacterium, it will be Gram Positive Rods or Gram Negative Rods. C. • If your Unknown is a Soil Isolate, it will be either Gram Positive Rods or Gram Negative Rods. • If your Unknown is Skin Isolate, it will be Gram Positive Cocci. • If your Unknown is an Enteric Bacterium, it will be Gram Positive Rods or Gram Negative Rods. D. • If your Unknown is a Soil Isolate, it will be either Gram Positive Rods or Gram Negative Rods. • If your Unknown is Skin Isolate, it will be Gram Positive Cocci. • If your Unknown is an Enteric Bacterium, it will be Gram Negative Rods. 5. Which Rogues Gallery Bacterium was growing on F Agar?
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MIC 101 Exam 1 Page 4 6. In Lab 2 you generated a Bacterial Growth Curve and determined the Doubling Time for E. coli by plotting the Optical Density of the Growth Medium at different Timepoints. A more accurate Method would be to determine the Number of Viable Cells in the Growth Medium at different Timepoints. You would inoculate your Clan ʼ s Flask with 500 μ l of E. coli at T= 0. At each Timepoint, you would take 500 μ l from your Flask in the 37°C Shaking Water Bath and transfer it to a Tube containing 4.5 ml of Sterile Growth Medium (10 -1 Dilution). You would then transfer 500 μ l from this 10 -1 Dilution Tube to 4.5 ml of Sterile Medium in your 10 -2 Tube. You ʼ d continue this Dilution Process until you had made 10 -1 through 10 -8 Dilutions for each Timepoint.
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