Technique for Adding a Stain when making a Wet Mount5.Turn on the microscope and place the slide on the stage; making sure the "e" is facing the normal reading position(see the figure above). Using the course focus and low power, move the
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body tube down until the "e" can be seen clearly. Draw what you seein your lab notebook. Be sure to include the magnification!6.Describe the relationship between what you see through the eyepiece and what you see on the stage. ________________________________________________________________________7. Looking through the eyepiece, move the slide to the upper right area of the stage.What direction does the image move?_________________________________________Now, move it to the lower left side of the stage. What direction does the image move?__________________________________________________________________________9. Re-center the slide and change the scope to high power. You will notice the "e" is out of focus. Do Not touch the coarse focus knob, instead use the fine focus to resolve the picture. Draw the image you see of the letter e (or part of it) on high power. Be sure to include the magnification!10.Locate the diaphragm under the stage.Move it and record the changes in light intensity as you do so. ________________________________________________________________________III. Determining Total Magnification:1.Locate the numbers on the eyepiece and the low power objective and fill in the blanks below. Ocular are 10x.Eyepiecemagnification______________(X)Objectivemagnification______________=TotalMagnification_____________X2.Do the same for the high power objective.Eyepiecemagnification______________(X)Objectivemagnification______________=TotalMagnification_____________X3. Write out the rule for determining total magnification of a compound microscope.
4.Obtain a prepared slide of an onion root tip. Focus and draw what yousee on low power and then re-focus and draw what you see on high power.Higher PowersCompound microscopes are parfocal; that is, once the object is in focus withlowest power, it should also be almost in focus with the higher power. 1.Bring the object into focus under the lowest power by following the instructions in the previous section. 2.Make sure that the letter eis centered in the field of the lowest objective. 3.Move to the next higher objective (low power, 10x) by turning the nosepiece until you hear or feel it click into place. Do not change the focus; parfocal microscope objectives will not hit normal slides when changing the focus if the lowest objective is intially in focus. 4.If any adjustment is needed use only the fine-adjustment knob. Alwaysuse only the fine-adjustment knob with higher powers. On your drawing of the letter ethat you made for exercise 1, draw a circle around the portion of the letter that you are now seeing with the higher-power magnification.
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