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Lecture6genetargetingHemophiliahumanchromosomes

Text questions anytime during the class to 858 360

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text questions anytime during the class to: 858-360-4528
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Problems with genes that act in multiple tissues, at multiple times How to activate the mutation at a time or in the tissue of choice? Solution: “conditional” knock-out, using microbial recombinase systems: Cre recombinase, recognises loxP sequences (originally found in P1 bacteriophage) FLP recombinase, recognizes FRT sequences (from yeast)
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Tissue-specific disruption of a gene using Cre/ LoxP system floxed gene “deleter” mouse, expresses cre recombinase: - Depending on the promoter to which Cre is fused, it can be expressed either in all cells, or just in a subset of cells (brain, liver, etc) For more information: http://cre.jax.org/introduction.html
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Tissue-specific disruption of a gene using Cre/LoxP system
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You set up a cross between the following kice: - A mouse in which an essential exon 5 of gene X is floxed (in both alleles of gene X - A mouse that is heterozygous for the deletion of gene X and contains a transgene of Cre recombinase under the control of a pancreas-specific promoter. Which of the following is TRUE regarding the progeny of this cross? A. Gene X will be expressed only in the pancreas B. Exon 5 will be deleted in all cells in half of the progeny C. Gene X will not be expressed in the pancreas of half of the progeny D. Cre recombinase will be deleted in one quarter of the progeny
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