Aeromonas veronii biovar sobria Aer veronii has been implicated as a potential

Aeromonas veronii biovar sobria aer veronii has been

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Aeromonas veronii biovar sobria Aer. veronii has been implicated as a potential fish pathogen but only in laboratory- based experiments, where intramuscular injection of 10^ cells/ml resulted in muscle necrosis in Atlantic salmon (Mcintosh and Austin, 1990b). In a subsequent study, identification was achieved after examination of 14 isolates by FAME and AFLP fingerprinting, and biochemical profiling using the API 20E, API 20NE and Phene- Plate system (Rahman et al., 2002a). Alteromonadaceae representative Pseudoalteromonas piscicida An isolate, coined Cura-d, from Amblyglyphidodon curacao eggs was identified by 16S rDNA sequencing as Pseudoalteromonas piscicida. Pseudoalteromonas piscicida Colonies on marine 2216E agar were 3-6 mm in diameter and orange to dark orange in colour (the centres were often white) with the pigment diffusing into the agar. Cells comprised oxidative. Gram-negative, polarly flagellated rods, which utilised fructose, maltose, mannose and sucrose, but not L-threonine,
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100 Bacterial Fish Pathogens and contained intracellular granules, but not poly-P-hydroxybutyrate. Growth occurs at 40°C (Nelson and Ghiorse, 1999). Shewanella putrefaciens During Spring of 1985, a disease occurred which resulted in high mortahties in rabbit- fish, Siganus rivulatus, farmed in sea cages in the Red Sea. From diseased animals, a Gram-negative bacterium was recovered, which was capable of re-infecting healthy fish (Saeed et al, 1987). To date, the disease has not been described in any other fish species, or, for that matter, elsewhere. Shewanella putrefaciens Cultures comprise motile Gram-negative rods, which are neither fermentative nor oxidative for glucose, but which grow at 15-42°C, in 0.85-9.0% (w/v) sodium chloride, at pH 6.2-9.6, and on MacConkey agar. Catalase, H2S, ornithine de- carboxylase and oxidase are produced, but not arginine dihydrolase, P-galactosi- dase, indole, lysine decarboxylase, or phenylalanine or tryptophan deaminase. Gelatin but not urea is attacked. Nitrates are reduced, and citrate is utilised. The Voges Proskauer reaction is negative. Acid is not produced from amygdalin, arabinose, glucose, inositol, mannitol, meUbiose, rhamnose, sorbitol or sucrose. Growth occurs at 37°C, and in 7% but not 0% (w/v) sodium chloride (Saeed et ai, 1987). From the results of the API 20E rapid identification system, it was considered that the pathogen was Ps. (= Alteromonas) putrefaciens, a taxon which has been subsequently re-classified as Shewanella putrefaciens (MacDonell and Colwell, 1985). Campylobacteriaceae representative Arcobacter cryaerophilus According to Aydin et al. (2002), the cultures were identified according to the results in Sergey's Manual of Systematic Bacteriology, and there does appear to be a close agreement in the characteristics. Arcobacter cryaerophilus The small, white colonies comprise aerobic. Gram-negative, motile rods that produce catalase and oxidase, but not alkaline phosphatase, arginine dihydrolase, H2S, indole or urease, reduce nitrates to nitrites, do not attack aesculin, gelatin, starch or Tween 20 or 80, grow at 14-42°C and in potassium cyanide, but not in 3%
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  • Spring '20
  • Bacteria, representative, gram-negative bacteria

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