Several different species including e coli secrete

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Several different Species -- including E. coli -- secrete Siderophores but Pseudomonas is one of only a Handful of Species that secretes Fluorescent Siderophores. Procedure 1. Sterilize your Transfer Loop in the Flame of your Bunsen Burner. 2. Cool your Sterilized Transfer Loop by touching it to a Portion of your Pseudomonas Plate without any Colonies. 3. Use your Transfer Loop to transfer a very small Amount of a Bacteria to your Labeled F Agar Plate. Streak this F Agar Plate exactly as you would any other Plate. 4. Incubate your F Agar Plate at 30°C for at least 48 Hours. ______________________________________ “So what are you supposed to be, a lion tamer?” ‘I'm allowing you to tag along. So why don't you give your mouth a rest. Okay doll?’ “What do you mean ‘tag along’? Ever since you got into my club, you haven't been able to take your eyes off of me.” ‘Oh, yeah?’ © 1984 LucasFilm, Ltd.
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Lab 6 Page 15 IMViC Materials (per Clan): • 1 Tryptone Broth Tube (White Cap, Chablis-colored Medium) • 1 MR-VP Tube (Stainless Steel Cap, MGD-colored Medium) • 1 Citrate Slant (White Cap, Coastal Redwoods-colored Agar Slant) • Inoculating Loop • Bunsen Burner • Plastic Cup • Your Enteric Isolate (Enterobacter aerogenes or Escherichia coli ). The IMViC only makes Sense when it is performed on Gram Negative, Oxidase Negative Bacteria. Labeling is extremely important. Put the necessary Information on a Piece of Tape, make a Quick Release Tab and then attach the Tape to the Tube. We’ll be splitting the Contents of the Incubated MR-VP Tubes in the next Lab, so you’re actually setting-up for 2 separate Biochemical Tests. Individual IMViC Test Procedures Indole 1. Sterilize your Transfer Loop in your Bunsen Burner Flame. 2. Cool your Sterilized Inoculating Loop on a Portion of your Enteric Isolate Plate (either Enterobacter or E. coli ) without any Colonies. 3. Use your Inoculation Loop to pick-up a very small Amount of Bacteria from the Edge of a well-isolated Colony of your Enteric Isolate. 4. Transfer these Bacteria to a Labeled Tube of Tryptone Broth. All you need to do is stick the Loop into Top of the Broth and move it up and down several Times at the Air-Liquid Interface. 5. Resterilize your Loop. 6. Place your labeled and inoculated Tryptone Broth Tube into a Plastic Cup and incubate it at 37°C for 48 Hours. _______________________________________________ “We weren't brought here. Our plane crashed.” ‘It crashed’. No, no, no. We prayed to Shiva to help us find the stone. It was Shiva who made you fall from sky. So you will go to Pankot Palace... and find Shivalinga... and bring back to us. Bring back to us. Bring back to us. © 1984 LucasFilm, Ltd.
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Lab 6 Page 16 MR-VP 1. Sterilize your Transfer Loop in your Bunsen Burner Flame. 2. Cool your Sterilized Inoculating Loop on a Portion of your Enteric Isolate Plate (either Enterobacter aerogenes or E. coli ) without any Colonies.
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