deionized water catechol potato juice extract cuvettes parafilm kimwipes and a

# Deionized water catechol potato juice extract

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deionized water, catechol, potato juice extract, cuvettes, parafilm, kimwipes, and a spectrophotometer. Label four blank as stated in table 3. The exact concentrations can be found in table 3. Label four of the test tubes according, to table 4. Set the wavelength to 420nm on the spectrophotometer. Put catechol to the first test tube and mix up the contents and immediately measure the absorbance. Start talking the absorbance measurements every 1 to 6 minutes. Repeat the procedure for the other three test tubes and create a graph with the best fit line. Table 3 Blank Test Tube Setup Test Tube pH Phosphate Buffer Potato Juice Water Total Volume Blank A 11 mL 0 mL 1 mL = 12 mL Blank B 10.5 mL 0.5 mL 1 mL = 12 mL Blank C 10 mL 1 mL 1 mL = 12 mL Blank D 9 mL 2 mL 1 mL = 12 mL Table 4 Experimental Test Tube Setup Test Tube pH Phosphate Buffer Potato Juice Catechol Total Volume A 11 mL 0 mL 1 mL = 12 mL B 10.5 mL 0.5 mL 1 mL = 12 mL C 10 mL 1 mL 1 mL = 12 mL D 9 mL 2 mL 1 mL = 12 mL Results The experiment showed the highest absorption of solution in experiment tube with pH8, it is 0.170 in (Table 5). At the pH of 4 the absorbance was 0.014, at a pH of 6 the absorbance was 0.020, at a pH of 7 the absorbance was 0.0.092, at a pH of 8 the absorbance was 0.170, and at a pH of 10 the absorbance was 0.033. Table 5 pH Effects on Enzyme Activity Absorbance at 420 nm pH 4 pH6 pH 7 pH 8 pH 10 0.014 0.020 0.092 0.170 0.033 Graph 1 4 7 11 14 0 0.045 0.09 0.135 0.18 pH Effect on Enzyme Activity Series1 pH Absorbance at 420 nm The second experiment reflected that the highest rate of reaction was in the solution with 2 mL of the enzyme added (Column D, Table 6). The data is also represented by graph 2. For experiment A at time the time of zero, the absorbance was 0.847. It started increasing at each minute after minute one which was 0.847, 0.848 at minute two, 0.849 at minute three 0.849 at minute four, 0.850 at minute five, and 0.850 at minute six. For experiment B at the time zero, the absorbance was 0.125. After this, it increased each minute to 0.145 at minute one, at minute 2 it was 0.165, at minute three it was 0.183, it was 0.195 at minute four, 0.1205 at minute five, and at minute six, it was 0.212. For experiment C at time 0 the absorbance was 0.067 and then increased each minute to 0.113 at minute 1, 0.148 at minute 2, 0.171 at minute 3, 0.186 at minute 4, 0.196 at minute 5, and 0.204 at minute 6. For experiment D at time 0 the absorbance was 0.63, and then it increased each minute to 0.163 at minute 1, 0.183 at minute 2, 0.214 at minute 3, 0.239 at minute 4, 0.250 at minute 5, and 0.260 at minute 6.  #### You've reached the end of your free preview.

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