33 C OMPARISONS OF C LINICALS AND BIOCHEMICALS PARAMETERS BETWEEN VDR P

33 c omparisons of c linicals and biochemicals

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3.3 C OMPARISONS OF C LINICALS AND BIOCHEMICALS PARAMETERS BETWEEN VDR P OLYMORPHISM We compared biochemical and clinical parameters between VDR genotypes for all patients and controls combined. The Fok-I Polymorphism was associated significantly with increased levels of TC, LDL-C HDL-C and TG (all P values <0.05). We found significantly elevated systolic blood pressure (P = 0.042) in association with Apa1 polymorphism. No Significant association was observed for Bsm1 polymorphism (Table 3). 3.4 A NALYSIS OF VDR H APLOTYPE The combined effect of three variants (Fok-I, Bsm-I, Apa-I) in the VDR gene were examined. There were 7 haplotypes identified in the VDR gene in our population, with frequencies greater than 5%. No significant association was observed between the all haplotypes and DT2M. Table 2. Genotypic distribution of VDR polymorphisms and statistic comparison between DT2 subjects and controls VDR rs10735810 (Fok-I) Model Genotype Controls (n= 177) Patients (n= 176) OR (95% CI) P-value Codominant FF 82 (46.33%) 87 (49.43%) Reference Ff 74 (41.81%) 80 (45.45%) 0.99(0.63-1.54) 0.958 ff 21 (11.86%) 9 (5.11%) 0.35(0.14-0.83) 0.018 Dominant FF 82 (46.33%) 87 (49.43%) Reference Ff+ff 95 (53.67%) 89 (50.57%) 0.84(0.55-1.28) 0.420 Recessive FF+Ff 156 (88.14%) 167 (94.89%) Reference ff 21 (11.86%) 9 (5.11%) 0.35(0.15-0.82) 0.016 VDR rs1544410 (Bsm-I) Model Genotype Controls (n= 177) Patients (n= 176) OR (95% C`I) P-value Codominant BB 18 (13.24%) 18 (13.33%) Reference Bb 57 (41.91%) 57 (42.22%) 1.00 (0.47-2.13) 1 bb 61 (44.85%) 60 (44.44%) 0.99 (0.46-2.10) 0.979 Dominant Bb 61 (44.85%) 60 (44.44%) Reference Bb+bb 75 (55.15%) 75 (55.56%) 0.99 (0.49-2.02) 0.989 Recessive bb+Bb 118 (86.76%) 117 (86.67%) Reference BB 18 (13.24%) 18 (13.33%) 0.98 (0.61-1.59) 0.951 VDR rs7975232 (Apa-I) Model Genotype Controls (n= 177) Patients (n= 176) OR (95% CI) P-value Codominant AA 36 (22.50%) 36 (22.64%) Reference Aa 90 (56.25%) 89 (55.97%) 0.99 (0.57-1.71) 0.965 aa 34 (21.25%) 34 (21.38%) 1.00 (0.50-1.98) 1 Dominant A/A 36 (22.50%) 36 (22.64%) Reference Aa+aa 124 (77.50%) 123 (77.36%) 0.99 (0.58-1.68) 0.971 Recessive A/A+Aa 126 (78.75%) 125 (78.62%) Reference aa 34 (21.25%) 34 (21.38%) 1.01 (0.58-1.74) 0.982
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Relationship between Vitamin D Receptor (VDR) Gene Polymorphisms and susceptibility to Type 2 Diabetes Mellitus in Moroccans population ISSN : 2028-9324 Vol. 8 No. 2, Sep. 2014 508 The frequencies of all haplotypes are listed in Table 5. The interaction between haplotypes and clinical and Biochemical’s parameters showed that H1 HAPLOTYPE was associated with higher waist circumference and lower triglycerides levels (TG). The H2 haplotype was associated significantly with lower HDL-C. No difference significant was detected between others Haplotypes (Table 6) Figure 1 The Linkage Disequilibrium (LD) between the three VDR SNPs 4 D ISCUSSION Vitamin D is reported to be involved in several biological processes. Variations in this vitamin D endocrine systems can lead to several common chronic diseases, such as cardiovascular disorders, diabetes, cancer, tuberculosis, and osteoarthrosis [13], [14]. The gene of VDR is one of the important genes that have been expressed in a large number of different tissues [13]. Pancreatic beta-cells is among the several types of cells that respond to vitamin D [14]. Thanks to this, VDR genotype can play an important role in glucose homeostasis and in the mechanism of insulin release [15]. We conducted this study aiming to clarify the contribution of VDR polymorphisms in susceptibility to T2DM among Moroccan Population. To our knowledge, this is the first study to studied the prevalence of Vitamin D inadequacy in Moroccan population with T2DM, and the distribution of VDR polymorphisms in relation biochemical’s and anthropometric parameters.
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