Mod1LabWorksheet_04082018.docx

4 view your slide under a microscope and utilize the

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4. View your slide under a microscope and utilize the oil immersion lens to provide a differentiation of the cells. 5. Note any observations you see within your prepared slide: Part 03 Procedure: Morphology of Blood Diseases and Disorders 1. Compare and contrast the concentration and morphology of red blood cells and any observations of the leukocytes and thrombocytes within the following prepared slides: A) NORMAL BLOOD SMEAR: Erythrocytes (size, color, shape and concentration): Majority of cells are biconcave, relatively flat disc shaped, red in color, heavy concentration. Leukocytes: Not many leukocytes appear, large and circular in appearance, bluish/ purple color Thrombocytes: Red in color, circular shape, appear to be smallest (Betts, 2017). B) IRON DEFICIENCY ANEMIA Erythrocytes (size, color, shape and concentration): Cells appear to be lighter in color, transparent through the middle, no change to size or shape Leukocytes: No apparent changes from normal smear Thrombocytes: No apparent changes from normal smear (Betts, 2017). C) SICKLE CELL ANEMIA Erythrocytes (size, color, shape and concentration): “Sickled” cells are warped in appearance, half circles or smooshed, not all cells appear this way, others are as they appear in a normal smear Leukocytes: Do not appear on this slide, no expected changes Thrombocytes: Not apparent on this slide, no expected changes (Betts, 2017). D) THALASSEMIA MAJOR Erythrocytes (size, color, shape and concentration): Smaller, misshaped not smashed like sickle cell, lighter in color and seemingly mor transparent through the center.
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Leukocytes: Present, similar in appearance to that of a normal smear. Thrombocytes: No apparent changes from normal smear (Betts, 2017). E) THROMBOCYTOSIS Erythrocytes (size, color, shape and concentration): Many more cells, appear clumped together in groups of two or three Leukocytes: Do not appear on this slide, no expected changes Thrombocytes: Increase in number, no change in appearance (Betts, 2017). Part 04 Procedure: Case Studies 1. Utilizing the case study hand out provided by your instructor, answer the following questions: Case Study A 1. What are some of the possible causes to Jackie’s symptoms? One common reason for her fatigue, SOB, and light-headedness could be palpations; however, chewing on ice or “pica” is a common sign of iron deficiency anemia. (Betts, 2017). 2. What test results are abnormal in Jackie’s CBC? What do they represent? Hgb (10.3): decreased Hgb can result in SOB or light-headedness due to the inability of RBCs to transport O2. MCV (63): decreased size or volume of RBCs could explain the lack of Hgb and result in similar symptoms. MCH (22.6): This is expected due to the low Hgb numbers as it is the average concentration of Hgb in RBCs. RDW (18): This number is high which may indicate a large variation in the size of RBCs, which could indicate anemia.
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