Once DNA synthesis has finished, it is important that the newly synthesised strands are bound andstabilized. With regards to the lagging strand, two enzymes are needed to achieve this;RNAaseHremoves the RNA primer that is at the beginning of each Okazaki fragment, andDNALigasejoins fragments together to create one complete strand.SUMMARYReplication in eukaryotes starts at multiple origins of replication. A primer is required to initiatesynthesis, which is then extended by DNA polymerase as it adds nucleotides one by one to thegrowing chain. The leading strand is synthesized continuously, whereas the lagging strand issynthesized in short stretches called Okazaki fragments. The RNA primers are replaced with DNAnucleotides; the DNA remains one continuous strand by linking the DNA fragments with DNAligase. Below is a summary table of the major enzymes addressed in this reading, listed in roughorder of activity during replication.Important Enzymes in DNA ReplicationEnzymeFunctionTopoisomeraseRelaxes the super-coiled DNADNA helicaseUnwinds the double helix at the replication forkPrimaseProvides the starting point for DNA polymerase to begin synthesisof the new strandDNApolymeraseSynthesizes the new DNA strand; also proofreads and correctssome errorsDNA ligaseRe-joins the two DNA strands into a double helix and joinsOkazaki fragments of the lagging strandWhat’s More6
Fill in the blanks to complete the complementary strand of DNA producedduring replication.1.GGTACTGAATTGAGATTACGT__________________________2.CTAAGGCGATTATAGGATGAA__________________________3.CAAGACCAGGTTATAGACCAT__________________________4.GTAACCCGATTATAGGATCAA__________________________5.TTTACTGCCTTGAGATTAGGC__________________________Answer the following questions.1.What is the first step in the process of DNA replication?___________________2.Which enzymes is responsible for bonding the nucleotides in a new DNA molecule?_______________________________________________________3.What are the three major steps of DNA Replication?