A protein called dnak will bind to promoters of heat

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a protein called DnaK will bind to promoters of heat shock genes and directly enhance gene expression D: a response is observed that is very similar to bacteria that are exposed to a drop in pH 6: When low ammonia (NH 3 ) concentrations are available in an E. coli cell: A: more of the enzyme glutamine synthetase (GlnA) is needed than under high ammonia conditions. B: NtrB phosphorylates NtrC, and therefore the NtrC~P concentration will be high. C: Ntr B~P binds to DNA sites upstream of the glnA operon and activates transcription. D: NtrC~P contacts RNAP containing sigma54 7: The helical repeat experiment with araBAD : A: provided evidence that positive regulation of araBAD involves DNA looping. B: provided evidence that negative regulation of araBAD involves DNA looping. C: showed that insertion of 5, 15 and 25 bp between araO2 and araI allowed nearly normal regulation. D: showed that insertion of 10, 20, and 30 bp between araO2 and araI allowed nearly normal regulation. Page 1, v2
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Microbial Genetics BIOL518 Exam 4 - Fall 2010 Name_____________________________________________________________________ 8: During the stationary phase response A: a small non coding RNA termed DsrA will bind to rpoS transcript and inhibit translation B: translation of rpoS occurs at a level similar to that during exponential growth C: an RNA binding protein termed Hfq will bind rpoS transcript and inhibit translation D: the majority of rpoS regulation (expression of RpoS) occurs prior to rpoS transcription 9: Which of the following contribute to the absence of transcription of heat shock genes (genes that are transcribed by RNA polymerase containing RpoH σ 32 ) when E. coli cells have NOT been exposed to elevated (high) temperatures? A: There is no transcription of the gene encoding σ 32 under these conditions. B: Binding of DnaK to σ 32 blocks the ability of σ 32 to interact with RNA polymerase core enzyme (or to cognate promoters). C: Much of the σ 32 protein will be degraded by the protease FtsH. D: The mRNA of the σ 32 transcript folds into a structure that reduces its translation. 10: In the presence of a large excess of free tryptophan, the E. coli trpEDCBA leader mRNA: A: will not be transcribed. B: will form the attenuator stem-loop (hairpin). C: will form the pause stem-loop and the attenuator stem-loop (hairpin). D: will form the anti-terminator stem-loop (hairpin). 11: During the regulation of virulence gene expression in Corynebacterium diptheriae , A: the decreased levels of iron in the host are a signal to activate expression of virulence genes B: the two component signal transduction system BvgS/BvgA is used to activate virulence gene expression C: expression of the bacteriophage encoded diptheria toxin is affected D: a transcriptional repressor (DtxR) inhibits expression of the diptheria toxin when iron is present 12: The sigma-54 subunit, complexed with RNA polymerase core enzyme: A: can form an open promoter complex, but can not initiate mRNA chain growth.
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