These two plasmids found in bacillus anthracis are

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These two Plasmids found in Bacillus anthracis are Classic Examples of -- A. Flexible Gene Pool Genes that encode for essential Proteins involved in Structure and Function. Flexible Gene Pool Genes have a Homogeneous G + C Content. B. Flexible Gene Pool Genes that encode for non-essential Proteins that may confer the Bacterium with specific Advantages under different Circumstances. Flexible Gene Pool Genes have a Heterogeneous G + C Content. C. Core Gene Pool Genes that encode for essential Proteins involved in Structure and Function. Core Gene Pool Genes have a Homogeneous G + C Content. D. Core Gene Pool Genes that encode for non-essential Proteins that may confer the Bacterium with specific Advantages under different Circumstances. Core Gene Pool Genes have a Heterogeneous G + C Content.
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MIC 101 Exam 1 Page 23 39. Transposition was first observed in Corn in the 1940s by Barbara McClintock (and this was before DNA was known to be a Double Helix). Unfortunately, her Corn Fields were close to the ever-popular Cold Spring Harbor Softball Fields and were frequently heedlessly overrun by Outfielders going after Fly Balls. She was awarded the Nobel Prize in 1983. The actual Details of Transposition were demonstrated using the Mind-Bending Powers of Bacterial Genetic Analysis. Transposable Elements do not have a Fixed Location in the Genome but can move from Place to Place within the Genome. One Strain of E. coli has five (5) Copies of one such Transposable Element. The only Gene in this Transposable Element is the Gene for encoding the Enzyme Transposase, so the only Way to track its Movement is when it happens to disrupt the Functioning of a Gene by its Insertion. This particular Transposable Genetic Element is -- A. • IS2, an Insertion Sequence. • IS2 is flanked by Inverted Repeats. • Inverted Repeats are flanked by Direct Repeats . GATTACA CGGTC.Transposase.CTGGCGATTACA CTAATCTGCCAG ............. GACCGCTAATGT _______________________________________________________ B. • IS2, an Insertion Sequence. • IS2 is flanked by Direct Repeats. • Inverted Repeats are flanked by Inverted Repeats . GATTACA CGGTC.Transposase.CGGTCACATTAG CTAATCTGCCAG ............. GCCAGTAATGTC _______________________________________________________ C. • Tn3, a Transposon. • Tn3 is flanked by Inverted Repeats. • Inverted Repeats are flanked by Direct Repeats . GATTACA CGGTC.Transposase.CTGGCGATTACA CTAATCTGCCAG ............. GACCGCTAATGT _______________________________________________________ D. • Tn3, a Transposon. • Tn3 is flanked by Direct Repeats. • Direct Repeats are flanked by Inverted Repeats . GATTACA CGGTC.Transposase.CGGTCACATTAG CTAATCTGCCAG ............. GCCAGTAATGTC
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MIC 101 Exam 1 Page 24 40. One of the Experiments in the BIO 429 Course (MCB) at The University of Michigan involved transforming E. coli with a Plasmid containing two different Antibiotic Resistance Genes, and then mapping the relative Location of these Genes on this Plasmid. A Typical Transformation Experiment Protocol was: (1) Pre-incubate E. coli Overnight in a High-Salt (CaCl 2 ) Solution at 0°C to make them Competent. (2) Incubate these Competent E. coli with the Plasmid for 15 Minutes.
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