III. Chapter 22.3: Linkage Mapping Via Crosses A. Section Learning Objectives: 1.Define molecular marker. 2.Explain the use of molecular markers in mapping studies. 3. Use the terms listed below to correctly explain concepts, assigned figures, and specified end-of- chapter questions. B. Important Terms: Molecular markers Polymorphic RFLP AFLP STR/microsatellites SNP STS C. Assigned Figures : 22.4 (Just understand the content), 22.5*, and 22.6* (There is a video lecture for this figure in the Blackboard resource folder). D. Assigned Tables : None E. Assigned Comprehension Questions: 1-3 1. A molecular marker is a _____ found at a specific site on a chromosome that has properties that allow it to be ______. Segment of DNA, uniquely identified using molecular tools 2. Which of the following is an example of a molecular marker? All of the above 3. To map the distance between molecular markers via crosses, the markers must be polymorphic F. Problem Sets and Insights – Assigned Solved Problems: S1, S3 S1: S3: G. Problem Sets and Insights – Assigned Conceptual Questions : C3 C3: Which of the following statements is true about molecular markers? A. All molecular markers are segments of DNA that carry specific genes B. A molecular marker is a segment of DNA that is found at a specific location in a genome C. We can follow the transmission of a molecular marker by analyzing a phenotype (i.e. the individual’s bodily characteristics) of offspring D. We can follow the transmission of molecular markers using molecular techniques such as gel electrophoresis E. An STS is a molecular marker H. Problem Sets and Insights – Assigned Experimental Questions: E11, E14, E18 5
E11. A woman has been married to two different men and produced five children. This group is analyzed with regard to three different STSs; STS-1, is 146 and 122 bp; STS-2 is 102 and 88 bp; and STS-3 os 188 and 204 bp. The mother is homozygous for all three STSs: STS-1= 122, STS-2 =88 and STS-3= 188. Father 1 is homozygous for STS-1= 122 and STS-2= 102, and heterozygous for STS-3= 188/204. Father 2 is heterozygous for STS-1=122/146, STS-2=88/102 and homozygous for STS- 3=204. The five children have the following results: Which children can you definitely assign to father 1 or father 2? Child 1 and child 3 belong to father 2; child 2 and child 4 belong to father 1; child 5 could belong to either father. E14. Take a look at solved problem S1. Lets suppose a male is heterozygous for two polymorphic sequence-tagged sites. STS-1 exists in two sizes: 211 bp and 289 bp. STS-2 also exists in two sizes: 115 bp and 422 bp. A sample of sperm was collected from this man, and individual sperm were placed into 30 separate tubes. Into each of the 30 tubes were added the primers that amplify STS-1 and STS-2, and then the samples were subjected to PCR. The following results were obtained.
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